Expression of Cbfα1/p56 subtype in bone marrow mesenchymal stem cells from rat mandible

doi:10.3969/j.issn.2095-4344.2014.23.003

Abstract

Abstract:

BACKGROUND: Unlike the ilium derived from the paraxial mesoderm, the mandible from cranial neural crest has a unique mechanism. Core binding factor α1 (Cbfα1) is a key transcription factor for skeletogenic process. However, the role of Cbfα1/p56 subtype in mandible tissue is yet not clear.

OBJECTIVE: To research the expression of Cbfα1/p56 subtype in bone marrow mesenchymal cells from rat mandible in vitro.

METHODS: Bone marrow mesenchymal stem cells from rat mandible and ilium were in vitro isolated and purified by primary culture. The characteristics of bone marrow mesenchymal cells were compared through the methods of enzyme linked immunosorbent assay and real-time PCR, including growth curve, alkaline phosphatase activity and relative mRNA expression of Cbfα1 subtypes.

RESULTS AND CONCLUSION: Bone marrow mesenchymal cells from rat mandible and ilium were successfully obtained. Bone marrow mesenchymal cells from the mandible proliferated more rapidly, alkaline phosphatase activity of which was higher than iliac cells. The relative mRNA expression of Cbfα1/p56 subtype in bone marrow mesenchymal cells from the mandible was more than that in iliac cells at 6 days of culture (P < 0.05), while the expression of Cbfα1/p57 in each time showed no statistical significance (P > 0.05). The results showed that Cbfα1/p56 is very significant in the early osteogenic differentiation of bone marrow mesenchymal cells from the mandible.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, ilium, mandible, core binding factor alpha 1 subunit

CLC Number:

R394.2

Zhou Jian-hua, Xu Yan-bin, Qiu Jian-zhong, Chen Zheng-gang, Jiang Hong-bing, Wang Li-li. Expression of Cbfα1/p56 subtype in bone marrow mesenchymal stem cells from rat mandible[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(23): 3622-3626.

Figures/Tables 1

References

[1] Rath-Deschner B, Daratsianos N, Dühr S,et al.The significance of RUNX2 in postnatal development of the mandibular condyle.J Orofac Orthop. 2010;71(1):17-31.
[2] Liu JC, Lengner CJ, Gaur T,et al. Runx2 protein expression utilizes the Runx2 P1 promoter to establish osteoprogenitor cell number for normal bone formation.J Biol Chem. 2011; 286(34):30057-30070.
[3] Henriquez B, Hepp M, Merino P,et al.C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells.J Cell Physiol. 2011;226(11): 3043-3052.
[4] Xiao Z, Awad HA, Liu S,et al.Selective Runx2-II deficiency leads to low-turnover osteopenia in adult mice.Dev Biol. 2005; 283(2):345-356.
[5] Vinardell T, Sheehy EJ, Buckley CT,et al.A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources.Tissue Eng Part A. 2012;18(11-12):1161-1170.
[6] 许艳彬,江宏兵,陈莉花,等.大鼠颌骨与髂骨来源骨髓基质干细胞的体外生物学特性比较[J].口腔医学, 2008,28(3):145-147.
[7] Brown JM, Nemeth K, Kushnir-Sukhov NM,et al.Bone marrow stromal cells inhibit mast cell function via a COX2-dependent mechanism.Clin Exp Allergy. 2011;41(4):526-534.
[8] Scherzed A, Hackenberg S, Froelich K,et al.BMSC enhance the survival of paclitaxel treated squamous cell carcinoma cells in vitro.Cancer Biol Ther. 2011;11(3):349-357.
[9] Livak KJ, Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Methods. 2001;25(4):402-408.
[10] Gimble JM, Nuttall ME.The relationship between adipose tissue and bone metabolism.Clin Biochem. 2012;45(12): 874-879.
[11] Potier E, Noailly J, Ito K.Directing bone marrow-derived stromal cell function with mechanics.J Biomech. 2010; 43(5): 807-817.
[12] Kuroda S.Bone marrow stromal cell transplantation for ischemic stroke -- its multi-functional feature.Acta Neurobiol Exp (Wars). 2013;73(1):57-65.
[13] Gimble JM, Nuttall ME. The relationship between adipose tissue and bone metabolism. Clin Biochem. 2012; 45(12): 874-879.
[14] Jeong H, Yim HW, Cho Y,et al.The effect of rigorous study design in the research of autologous bone marrow-derived mononuclear cell transfer in patients with acute myocardial infarction.Stem Cell Res Ther. 2013;4(4):82.
[15] Mostafa NZ, Uluda? H, Varkey M, et al. In vitro osteogenic induction of human gingival fibroblasts for bone regeneration. Open Dent. 2011;5:139-145.
[16] Kyllönen L, Haimi S, Mannerström B, et al. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro. Stem Cell Res Ther. 2013;4(1):17.
[17] Benoit DS, Boutin ME. Controlling mesenchymal stem cell gene expression using polymer-mediated delivery of siRNA. Biomacromolecules. 2012;13(11):3841-3849.
[18] Coelho MJ, Fernandes MH. Human bone cell cultures in biocompatibility testing. Part Ⅱ: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation. Biomaterials. 2000;21(11):1095-1102.
[19] Fiorentini E, Granchi D, Leonardi E, et al. Effects of osteogenic differentiation inducers on in vitro expanded adult mesenchymal stromal cells. Int J Artif Organs. 2011; 34(10): 998-1011.
[20] Kim M, Kim C, Choi YS, et al. Age-related alterations in mesenchymal stem cells related to shift in differentiation from osteogenic to adipogenic potential: implication to age-associated bone diseases and defects. Mech Ageing Dev. 2012;133(5):215-225.
[21] 庚佳佳,汪新柱,赵琳,等.兔骨髓间充质干细胞的分离培养及成骨诱导[J].中国组织工程研究, 2013,17(6):974-979.
[22] Kim H, Kim HM, Jang JE,et al.Osteogenic Differentiation of Bone Marrow Stem Cell in Poly(Lactic-co-Glycolic Acid) Scaffold Loaded Various Ratio of Hydroxyapatite.Int J Stem Cells. 2013;6(1):67-74.
[23] Akintoye SO, Lam T, Shi S,et al.Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals.Bone. 2006;38(6): 758-768.
[24] Rath-Deschner B, Daratsianos N, Dühr S,et al.The significance of RUNX2 in postnatal development of the mandibular condyle.J Orofac Orthop. 2010;71(1):17-31.
[25] Fang CY, Xue JJ, Tan L,et al.A novel single-base deletion mutation of the RUNX2 gene in a Chinese family with cleidocranial dysplasia.Genet Mol Res. 2011;10(4): 3539-3544.
[26] Ott CE, Leschik G, Trotier F,et al.Deletions of the RUNX2 gene are present in about 10% of individuals with cleidocranial dysplasia.Hum Mutat. 2010;31(8):E1587-1593.
[27] D'Alessandro G, Tagariello T, Piana G.Craniofacial changes and treatment of the stomatognathic system in subjects with Cleidocranial dysplasia.Eur J Paediatr Dent. 2010;11(1): 39-43.
[28] Zhang S, Xiao Z, Luo J,et al.Dose-dependent effects of Runx2 on bone development.J Bone Miner Res. 2009;24(11): 1889-1904.
[29] Jonason JH, Xiao G, Zhang M,et al.Post-translational Regulation of Runx2 in Bone and Cartilage.J Dent Res. 2009; 88(8):693-703.
[30] Fazenda C, Simões B, Kelsh RN,et al.Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Gene. 2010;450(1-2):94-102.
[31] Komori T.Regulation of bone development and maintenance by Runx2.Front Biosci. 2008;13:898-903.
[32] Xiao ZS, Hjelmeland AB, Quarles LD.Selective deficiency of the "bone-related" Runx2-II unexpectedly preserves osteoblast-mediated skeletogenesis.J Biol Chem. 2004; 279(19): 20307-20313.
[33] Santagati F, Rijli FM.Cranial neural crest and the building of the vertebrate head.Nat Rev Neurosci. 2003;4(10):806-818.
[34] Hunter MP, Prince VE.Zebrafish hox paralogue group 2 genes function redundantly as selector genes to pattern the second pharyngeal arch.Dev Biol. 2002;247(2):367-389.
[35] 周建华,陶震江,江宏兵,等.Hoxa2基因在大鼠颌骨及胫骨缺损再生修复中表达的比较研究[J].口腔医学,2007,27(4):193-195.