Expression of vascular endothelial growth factor in bone marrow mesenchymal stem cells under hypoxic conditions

doi:10.3969/j.issn.2095-4344.2014.23.004

Abstract

Abstract:

BACKGROUND: Whether transplanted bone marrow mesenchymal stem cells under hypoxic conditions can survive is crucial for the successful cell transplantation. Therefore, studies on the growth of bone marrow mesenchymal stem cells under hypoxic conditions in vitro can provide experimental evidence for in vivo cell transplantation.

OBJECTIVE: To observe the expression of vascular endothelial growth factor in bone marrow mesenchymal stem cells under hypoxia.

METHODS: Rat bone marrow mesenchymal stem cells were obtained and cultured, and observed under light microscopy. Passage 3 cells were cultured under normoxia (21% O₂) and hypoxia (3% O₂), respectively, for 72 hours. Then cell counting kit-8 assay and flow cytometry were employed to detect cell proliferation in the two groups. Western blot assay was adopted to detect the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor in the two groups.

RESULTS AND CONCLUSION: (1)Rat bone marrow mesenchymal stem cells were obtained and cultured successfully, which were fusiform cells and had uniform shape under the light microscope. (2)The results of cell counting kit-8 assay showed that the number of cells in the hypoxic group was higher than that in the normoxic group at each time point, and cell viability increased significantly at hours 36 and 48 (P < 0.05). (3)The results of flow cytometry demonstrated that the proportion of cells in S phase and cell proliferation index in the hypoxic group were significantly increased, compared with the normoxic group (P < 0.05). (4)Western blot results showed that there was a small amount of the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor in the normoxic group, but the expression of these two proteins in the hypoxic group was increased in a time-dependent manner (P < 0.05). These findings suggest that hypoxia can induce proliferation of rat bone marrow mesenchymal stem cells cultured in vitro, and also raise hypoxia-inducible factor-1α and vascular endothelial growth factor expression in a time-dependent manner.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, anoxia, hypoxia-inducible factor, alpha subunit, vascular endothelial growth factors

CLC Number:

R394.2

Guo Hui, Zhang Yu-juan, Wei Xiao-guang, Xu Biao, Chen Yong-zhen. Expression of vascular endothelial growth factor in bone marrow mesenchymal stem cells under hypoxic conditions[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(23): 3627-3632.

Figures/Tables 3

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