Effects of different implant methods on in vitro adhesion of tissue-engineered gingival cells

doi:10.3969/j.issn.2095-4344.2014.21.004

Abstract

Abstract:

BACKGROUND: How to construct tissue-engineered periodontal tissue through efficient combination of scaffold materials and seed cells in vitro is an important direction of current research.
OBJECTIVE: To compare the cell seeding efficiency of traditional static seeding method and collagen enclose seeding method to select a more appropriate seeding method for cell attach.
METHODS: Gingival fibroblasts from dogs at certain concentrations were seeded onto polylactic acid-chitosan- gelatin scaffolds with gradient and uniform pore sizes using the traditional static seeding method and collagen enclose seeding method, respectively. The number of adherent cells was counted to calculate the seeding efficiency for data comparison.
RESULTS AND CONCLUSION: The cell seeding efficiency of the collagen enclose seeding method was higher than that of the traditional static seeding method (P < 0.01). The collagen enclose seeding method can significantly improve the seeding efficiency and increase cell initial concentration on the scaffold, and this method can be used for gingival tissue engineering.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: vaccination, gingival, cells, cell adhesion, stents, fibroblasts

CLC Number:

R318

Zhong Dai-qin, Zhou Qin-hu, Jin Chen-xin, Liang Yan. Effects of different implant methods on in vitro adhesion of tissue-engineered gingival cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3299-3303.

Figures/Tables 3

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