The expression of inflammatory factors in different periods after acute spinal cord injury in rats

doi:10.3969/j.issn.2095-4344.2014.18.004

Abstract

Abstract:

BACKGROUND: With the development of modern transportation and mining industrial, spinal cord injury has become a common clinical frequently-occurring disease which was serious influence human physical health and quality of life.

OBJECTIVE: To investigate the inflammatory factors expression of interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-17A (IL-17A) in the rat model of acute spinal cord injury via phosphorylated signal transducer and activator of transcription 3 (p-STAT3) and explore the role of interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-17A (IL-17A) in the acute inflammatory reaction after spinal cord injury.

METHODS: A total of 75 adult male SD rats were divided into two groups, the sham operation group and the spinal cord injury group; then put them into the subgroups randomly, which were 1h, 6h, 24h and 72h (n = 15) Acute spinal cord injury rat model was established by the modified Alleng weight drop method. The sham operation group just underwent laminectomy. The rats were sacrificed at corresponding time after operation and spinal cord, spleen tissues were collected. Immunohistochemical used to detect the expressions of IL-6 and IL-17 in spinal cord tissue injury segment dynamic changes, mRNA expression of IL-1β, IL-6 and IL-17A were detected by RT-PCR, Western blot used to detect the expressions of p-STAT3 dynamic changes.

RESULTS AND CONCLUSION: The average expression level of p-STAT3, IL-1β, IL-6, and IL-17 in SCI group had significant increased from those in the sham operation group. The expression of inflammatory cytokines IL-1β and IL-6 immediately increased following injured and peaked at 6 h; the expression of IL-17 and p-STAT3 immediately increased and peaked at 24 h; then sluggishly decreased, but its expression of 72h after injury still more than the sham operation group(P <0. 05). The expression of pro-inflammatory cytokines IL-1β and IL-6 had significant increased following early injured via p-STAT3 may be play a role in secondary spinal cord injury by inflammatory cascade; abnormally elevated pro-inflammatory cytokines IL-17, possibly in secondary injury plays an important role in inflammation.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: spinal cord injuries, interleukin-1 beta, interleukin-6, interleukin-17

CLC Number:

R318

Zhong Shao-hui, Fang Ye, Peng Jin-zhen, Gao Tai-hang. The expression of inflammatory factors in different periods after acute spinal cord injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(18): 2806-2811.

Figures/Tables 3

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