Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (8): 1188-1194.doi: 10.3969/j.issn.2095-4344.2014
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Effect of Psammosilene gavage on the expression of
apoptosis-related proteins in chondrocytes of a rabbit osteoarthritis model
Peng Xu1, Yang Jibin2, You Qi2, Jin Ying2, Zhang Jun2, Ge Zhen2, Zou Gang2, Jiang Kongjun2, Liu Yi2
Received:
2019-05-07
Revised:
2019-05-21
Accepted:
2019-06-27
Online:
2020-03-18
Published:
2020-01-20
Contact:
Liu Yi, Professor, Master’s supervisor, First Department of Orthopedics, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
About author:
Peng Xu, Master, Department of Joint Surgery, Suining Central Hospital, Suining 629000, Sichuan Province, China
Supported by:
CLC Number:
Peng Xu, Yang Jibin, You Qi, Jin Ying, Zhang Jun, Ge Zhen, Zou Gang, Jiang Kongjun, Liu Yi.
Effect of Psammosilene gavage on the expression of
apoptosis-related proteins in chondrocytes of a rabbit osteoarthritis model
空白对照组:兔精神状态良好,食欲正常,活动自如,右侧后膝关节无肿胀、畸形,毛发光亮,体质量无减轻。股骨髁及胫骨平台软骨面色泽正常,表面光整,无骨赘。 骨关节炎模型组:兔精神状态、食欲差,造模后随时间推移活动逐渐受限,右膝关节出现肿胀,跛行,毛发稀疏暗淡,体质量减轻。关节软骨色泽灰暗,表面溃疡形成,可见大量骨赘形成,部分关节面软骨缺失,软骨下骨裸露。 金骨莲灌胃组:兔精神状态较好,食欲较正常,关节活动无明显受限,膝关节无明显肿胀,毛发较光亮,体质量无减轻。关节软骨色泽稍变暗,关节面粗糙,未见骨赘及软骨下骨裸露。 p38抑制剂组:兔精神状态、食欲较差,关节轻度肿胀。关节面粗糙伴糜烂,可见少量骨赘形成,未见明显软骨下骨裸露。 2.1.2 各组软骨组织学检查结果 关节软骨行病理切片苏木精-伊红及番红O染色观察,见图2。 "
空白对照组:软骨结构完整,软骨细胞排列整齐,在钙化层及辐射层之间可见到完整的细胞潮线。番红O染色观察到细胞基质正常染色。 骨关节炎模型组:软骨变薄,软骨4层结构不完整,结构紊乱,裂隙深,可见放射带,软骨细胞明显减少,潮线破坏。番红O染色观察到细胞基质失染。 金骨莲灌胃组:软骨浅表层不规则,软骨细胞弥漫性增生,细胞潮线可见。番红O染色观察到细胞基质轻度失染。 p38抑制剂组:软骨变薄,软骨细胞局部增生,细胞潮线欠完整,番红O染色观察到细胞基质中度失染。 2.2 各组软骨Pelletier及Mankin评分 骨关节炎模型组Pelletier及Mankin评分较金骨莲灌胃组及p38抑制剂组明显升高(P < 0.05),金骨莲灌胃组Pelletier及Mankin评分较p38抑制剂组明显降低(P < 0.05),见表2,图3。 "
2.3.2 各组软骨组织中Bax mRNA的相对表达量 药物干预8周后,金骨莲灌胃组软骨组织中Bax mRNA的相对表达量较骨关节炎模型组及p38抑制剂组均明显降低(P < 0.05);与骨关节炎模型组相比,p38抑制剂组Bax mRNA的相对表达量明显降低(P < 0.05),见表3和图4。 2.3.3 各组软骨组织中Cleaved Caspase-3蛋白的表达量 药物干预8周后,金骨莲灌胃组Cleaved Caspase-3蛋白的表达量较骨关节炎模型组及p38抑制剂组均明显降低(P < 0.05);与骨关节炎模型组相比,p38抑制剂组Cleaved Caspase-3蛋白的表达量明显降低(P < 0.05),见图5。 "
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