In vitro constructing artificial biomimetic periosteum

doi:10.3969/j.issn.2095-4344.2013.42.002

Abstract

Abstract:

BACKGROUND: The small intestinal submucosa has good biocompatibility and biodegradability, and also contains a variety of growth factors that can significantly promote cell adhesion, proliferation and differentiation. Currently, the small intestinal submucosa has been widely used in bone and cartilage, blood vessels, skin, bladder, smooth muscle and pancreatic tissue repair, showing good performance as a tissue-engineered cell scaffold.
OBJECTIVE: To investigate the in vitro feasibility of tissue engineered periosteum constructed by porcine small intestinal submucosa and osteoblasts differentiated from bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells were harvested from 2-week-old healthy New Zealand rabbits by using adherent method, and then cells were cultured, induced, differentiated and identified in vitro. Following induced differentiation and identification, the bone marrow mesenchymal stem cells were compounded with porcine small intestinal submucosa to fabricate tissue engineered periosteum. The adhesion, growth, and proliferation of cells on the materials were observed.
RESULTS AND CONCLUSION: At 5 days after inoculation, the cells receiving osteogenic induction could quickly adhere and proliferate on the surface of porcine small intestinal submucosa and be interconnected; at 10 days, the desmosomes formed among the cells, cell processes from osteoblasts were visible and attached to the small intestine submucosa; at 15 days, cell proliferation and secretion of matrix appeared, and multi-layer membrane-like structure formed on the surface of the small intestine submucosa. These findings indicate that after osteogenic induction, the bone marrow mesenchymal stem cells can be combined with porcine small intestinal submucosa to construct a tissue engineered periosteum, which is hoped to be an ideal scaffold for tissue engineering.

Key words: tissue engineering, osteoblasts, biomimetic materials, stem cells

CLC Number:

R318

Sun Rui, Chen Xiao-fei, Zhao Lin, Zhao Jian-hong, Yu Jia-jia, Ren Guang-tie, Tuo Zhen-he. In vitro constructing artificial biomimetic periosteum[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(42): 7349-7355.

Figures/Tables 5

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