Fabrication and properties of low-crystallinity carbonate apatite monolith bone graft

doi:10.3969/j.issn.2095-4344.2013.34.002

Abstract

Abstract:

BACKGROUND: As a good candidate for bioresorbable bone graft, carbonate apatite monolith can be prepared by sintering procedure; however, sintering can cause carbonate loss and result in a much lower rate of biodegradation compared to the human bone, thereby influencing the formation of new bone.
OBJECTIVE: To fabricate low-crystallinity carbonate apatite monolith as bone graft and test its properties.
METHODS: Calcium carbonate monolith prepared by carbonation of Ca(OH)₂ monolith was treated in 1 mol/L ammonium dihydrogen phosphate solution at 60 ℃ up to 14 days. Diametral tensile strength was examined for biomechanical properties; X-ray diffraction analysis, Fourier transform infrared spectroscopy, scanning electron microscope observation and chemical analyses (carbonate, calcium and phosphate content) were also performed for physical and chemical properties.
RESULTS AND CONCLUSION: The calcium carbonate completely transformed into low-crystallinity B-type carbonate apatite after treated for 14 days. Diametral tensile strength of the final product was (10.27±1.08) MPa, which is adequate as a reconstruction material for bone defect. The carbonate content was (4.80±0.50)%, similar to that of the nature bone. The molar Ca/P was 1.63±0.01, indicating the Ca-deficient carbonate apatite is obtained. The present method allows an easy fabrication of low-crystallinity B-type carbonate apatite block with adequate strength and without sintering process.

Key words: biomaterials, tissue-engineered bone materials, bone graft, carbonate apatite, calcium carbonate, ammonium dihydrogen phosphate, biomechanics, low-crystallinity

CLC Number:

R318

Lin Xin, Hou Min. Fabrication and properties of low-crystallinity carbonate apatite monolith bone graft[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6067-3072.

Figures/Tables 4

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