Cytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material

doi:10.3969/j.issn.2095-4344.2013.33.015

Abstract

Abstract:

BACKGROUND: Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material.
OBJECTIVE: To determine the potential toxic effects of ostrich corneal stromal scaffold on cells.
METHODS: Cell culture methods were used to culture L-929 cells in the extracts of ostrich acellular corneal stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cells after cultured for 1, 2 and 3 days.
RESULTS AND CONCLUSION: After the cells were cultured in the extracts of ostrich acellular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cells was graded as level 1. The cytotoxicity test was conducted according to the “National Standard of the People's Republic of China GB/T16886.5-2003”. After cultured in the extracts of ostrich acellular corneal stroma, a small number of cells were round in shape and loosely adherent without intracytoplasmic granules, and cell lysis could be observed occasionally. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that the ostrich acellular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.

Key words: tissue construction, cytology experiment in tissue construction, tissue-engineered cornea, cornea, ostrich, acellular corneal stroma, scaffold, dehydration, preservation, cytotoxicity test, provincial grants-supported paper

CLC Number:

R318

Liu Xian-ning, Zhu Xiu-ping, Wu Jie, Wang Li-fang, Yin Yong . Cytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.33.015.

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