Periodontal ligament cells from miniature swine grow on a hydroxyapatite scaffold

doi:10.3969/j.issn.2095-4344.2013.29.005

Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (29): 5290-5295.doi: 10.3969/j.issn.2095-4344.2013.29.005

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Periodontal ligament cells from miniature swine grow on a hydroxyapatite scaffold

Ma Lu-ping¹, Zhong Liang-jun², Zhang Yuan-ming³, Zhang Yuan⁴, Zhang Peng-tao⁴

¹Department of Stomatology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; ²Department of Stomatology, Clinical College of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China; ³Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; ⁴Department of Stomatology, Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China

Received:2013-04-16 Revised:2013-06-07 Online:2013-07-22 Published:2013-07-22
Contact: Zhang Yuan-ming, Professor, Doctoral supervisor, Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zymdxx@163.com
About author:Ma Lu-ping★, Studying for master’s degree, Department of Stomatology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China yayamlp@qq.com
Supported by:
Project of Science and Technology Supporting to Xinjiang Uygur Autonomous Region, No. 201091144*

Abstract

Abstract:

BACKGROUND: Periodontal tissue engineering technology provides new ideas and new ways for periodontitis-induced bone defect repair.
OBJECTIVE: To develop a culture model for the periodontal ligament cells of miniature swine, which was constructed with hydroxyapatite, to investigate the biocompatibility with hydroxyapatite.
METHODS: Periodontal ligament cells from miniature swine were harvested by using tissue explant method. Immunofluorescence was used to detect the expression of stromal cell antigen 1 in the periodontal ligament cells of miniature swine. The third passage cells were co-cultured with a three-dimension hydroxyapatite scaffold, and the biological characteristics of the cells were observed under a scanning electron microscope at days 1, 3, 7 of co-culture.
RESULTS AND CONCLUSION: The pirmary miniature swine periodontal ligament cells grew well, and they were positive for stromal cell antigen 1. Under the scanning electron microscope, the periodontal ligament cells of miniature swine grew well on the hydroxyapatite scaffold at days 1, 3, 7 of co-culture. These prove that the miniature swine periodontal ligament cells, which can be separated using tissue explant method and cultured successfully in vitro, can grow well on the hydroxyapatite scaffold.

Key words: biomaterials, tissue-engineered oral materials, periodontal ligament cells, in vitro culture, hydroxyapatite, scanning electron microscope, miniature swine, provincial grants-supported paper

CLC Number:

R318

Ma Lu-ping, Zhong Liang-jun, Zhang Yuan-ming, Zhang Yuan, Zhang Peng-tao. Periodontal ligament cells from miniature swine grow on a hydroxyapatite scaffold[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(29): 5290-5295.

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Periodontal ligament cells from miniature swine grow on a hydroxyapatite scaffold

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