Human placental mesenchymal stem cells promote angiogenesis

doi:10.3969/j.issn.2095-4344.2013.23.006

Abstract

Abstract:

BACKGROUND: The blood supply in seed cells and material compounds is the important issue in the construction of tissue engineered bone tissue. Placental mesenchymal stem cells are the potential seed cells in tissue engineering research, and it is of significance to study its differentiation into vascular endothelial cells as well as promotion of angiogenesis.
OBJECTIVE: To explore the effects of placental mesenchymal stem cells differentiating into vascular endothelial cells in vitro and promoting angiogenesis in vivo.
METHODS: Human placental mesenchymal stem cells were isolated and cultured. After the cell surface antigens were identified, the placental mesenchymal stem cells were induced with vascular endothelial growth factor and human basic fibroblast growth factor to differentiate into vascular endothelial cells in vitro. Then immunofluorescence staining for endothelial-specific markers KDR and the von Will brand factor were used to characterize the cells after induction. Eight healthy adult New Zealand rabbits were prepared for 1.5-cm defect models in the middle segment of radius. Then the models were implanted with human placental mesenchymal stem cells/silk fibroin/hydroxyapatite while those implanted with silk fibroin/hydroxyapatite were taken as the controls. At weeks 4 and 12 after operation, gross observation, histological observation and X-ray examination of implanted bone were performed to evaluate bone defects healing and angiogenesis.
RESULTS AND CONCLUSION: The morphology of induced placental mesenchymal stem cells was obviously changed, showing cell body contraction and enhanced stereognosis. Immunofluorescence staining analysis showed a positivity for endothelial-specific markers KDR and the von Will brand factor. After the human placental mesenchymal stem cells were cultured with silk fibroin/hydroxyapatite compound, new bone formation was visible at 4 weeks, and lamellar bone formed, trabeculation and neovascularization were detected at 12 weeks after implantation. While scaffold materials degraded gradually and no neovascularization was observed in the control group. Experimental findings indicate that, placental mesenchymal stem cells can differentiate into vascular endothelial cells in vitro, and combined transplantation with silk fibroin/hydroxyapatite can promote angiogenesis and repair bone defects.

Key words: stem cells, stem cell culture and differentiation, placental mesenchymal stem cells, silk fibroin, hydroxyapatite, bone defects, vascular endothelial cells, angiogenesis, scaffold, middle radius, vasc ular regeneration, tissue engineering, stem cell photographs-containing paper

CLC Number:

R394.2

Zhao Ji-dong, Miao Zong-ning, Qian Han-guang, Peng Wei, Si Zhi-ping. Human placental mesenchymal stem cells promote angiogenesis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(23): 4216-4233.

Figures/Tables 3

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