Effect of Gushining capsule at different doses on the microcirculation of rats with steroid-induced avascular osteonecrosis of the femoral head

doi:10.3969/j.issn.2095-4344.2013.20.016

Abstract

Abstract:

BACKGROUND: Gushining capsule is summarized as the effective prescriptions for steroid-induced avascular necrosis of the femoral head, formulated by Professor Ding E from Anhui University of Traditional Chinese Medicine after long-term clinical practice. The main principle of Gushining capsule is to disperse stagnation, reduce the superabundance, supplement the insufficiency, promote blood circulation, remove blood stasis, and engender new blood.
OBJECTIVE: To observe the microcirculation of steroid-induced avascular necrosis of the femoral head in the rabbits after Gushining capsule treatment.
METHODS: Six white rabbits were randomly selected as the normal control group. The model of steroid-induced femoral head avascular necrosis was constructed in 90 rabbits with the modified method using Escherichia coli endotoxin plus methylprednisolone, and model rats were randomly divided into: high-dose group of Gushining capsule, middle-dose group of Gushining capsule, low-dose group of Gushining capsule, model group and Xianlinggubao capsule control group. Rabbits in each group were killed at 4, 8, and 12 weeks after treatment, changes of endothelin, nitrogen monoxidum, vascular endothelial growth factor and histopathology were detected.
RESULTS AND CONCLUSION: Both Gushining capsule and Xianlinggubao capsule could increase endothelin and nitrogen monoxidum concentrations. Histopathological results showed that, compared with the model group, the severity of femoral head necrosis was significantly reduced in the Gushining capsule treatment groups, and vascular endothelial growth factor expression was significantly increased (P < 0.01 or P < 0.05). Among different dose groups of Gushining capsule, the middle-dose group achieved significant differences (P < 0.01 or P < 0.05), which was close to the detection levels in normal control group. Gushining capsules at middle dose can effectively improve the microcirculation status of steroid-induced femoral head necrosis.

Key words: tissue construction, tissue construction and traditional Chinese medicine, steroid-induced femoral head necrosis, Gushining capsule, Xianlinggubao capsule, endothelin, nitrogen monoxidum, vascular endothelial growth factor, Escherichia coli endotoxin, vascular density, vessel count, histology, provincial grants-supported paper

CLC Number:

R318

Li Chun-feng, Sun Zhi-tao, Zhou Zheng-xin. Effect of Gushining capsule at different doses on the microcirculation of rats with steroid-induced avascular osteonecrosis of the femoral head[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3723-3729.

Figures/Tables 5

References

[1] Fink B, Rüther W. Partial and total joint replacement in femur head necrosis. Orthopade. 2000;29(5):449-456.

[2] Dudkiewicz I, Covo A, Salai M, et al. Total hip arthroplasty after avascular necrosis of the femoral head: does etiology affect the results? Arch Orthop Trauma Surg. 2004; 124(2): 82-85.

[3] Folkman J, lngber DE,. Angiostatic steroids. Method of discovery andmechanism of action. Ann Surg 1987;206(3): 374.

[4] Nishimura T, Matsumoto T, Nishino M,et al. Hlistopathologic study of veins insteroid treated rabbits. Clin Orthop 1997; (334): 37-42.

[5] Bai LC, Li BQ, Ding E, et al. Anhui Zhongyi Xueyuan Xuebao. 2005;24(6):22-24.白良川,李保泉,丁锷,等.骨蚀宁对激素性股骨头坏死家兔局部rBF、TNF及显微结构的影响[J].安徽中医学院学报,2005, 24(6):22-24.

[6] He W, Xu CY, Fan YG, et al. Zhongguo Gushang. 2002;15(9): 531-533.何伟,徐传毅,樊粤光,等.活血化瘀中药对激素性股骨头坏死血浆TXB2与6-keto-PGF1α影响的实验研究[J].中国骨伤,2002, 15(9): 531-533.

[7] Sun ZT, Zhou ZX, Niu W, et al. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(7):1295-1298.孙志涛,周正新,牛维,等.骨蚀宁胶囊对激素性股骨头坏死模型兔凝血机制的影响[J].中国组织工程研究,2012,16(7):1295-1298.

[8] Jones JP Jr. Fat embolism, intravascular coagulation, and osteonecrosis. Clin Orthop Relat Res. 1993;(292):294-308.

[9] Wang GJ, Dughman SS, Reger SI, et al. The effect of core decompression on femoral head blood flow in steroid-induced avascular necrosis of the femoral head. J Bone Joint Surg Am. 1985;67(1):121-124.

[10] Watanabe Y, Terashima Y, Takenaka N, et al. Prediction of avascular necrosis of the femoral head by measuring intramedullary oxygen tension after femoral neck fracture. J Orthop Trauma. 2007;21(7):456-461.

[11] Vaishya R. Spontaneous fracture of the femoral neck in preexisting avascular necrosis of femoral head in sickle cell disease. Chin J Traumatol. 2012;15(5):312-314.

[12] Seipolt B, Dinger J, Rupprecht E. Osteonecrosis after meningococcemia and disseminated intravascular coagulation. Pediatr Infect Dis J. 2003;22(11):1021-1022.

[13] Wang Y, Gao CJ, Yang JC, et al. Zhongugo Bingli Shengli Zazhi. 2009;25(11):2233-2234,2239.王泳,高春锦,杨晋才,等.兔激素性股骨头坏死模型的建立[J].中国病理生理杂志,2009,25(11):2233-2234,2239.

[14] Zhao JD, Lv SC, Xue Z, et al. Zhongguo Guzhongliu Gubing. 2007;6(4):220-225.赵金东,吕松岑,薛震,等.激素性股骨头坏死动物模型的建立与评价[J].中国骨肿瘤骨病,2007,6(4):220-225.

[15] Zhou ZX, Liu AP, Wang F, et al. Zhongyiyao Linchuang Zazhi. 2007;19(3):209-210.周正新,刘安平,王峰,等.丁锷论治股骨头缺血性坏死的学术特点[J].中医药临床杂志,2007,19(3):209-210.

[16] Wang Z, Wang F, Zhang JH, et al. Anhui ZHongyi Xueyuan Xuebao. 2011;30(6):26-28.王正,王峰,张建华,等. 骨蚀宁胶囊配合髓芯减压植骨术治疗非创伤性股骨头缺血性坏死临床研究[J]. 安徽中医学院学报, 2011, 30(6):26-28.

[17] Zhang GL, Zhang M. Therapeutic progress of avascular osteonecrosis of the femoral head using a fibular graft by vascular anastomosis. Zhongguo Gu Shang. 2009;22(1): 76-78.

[18] Zalavras C, Dailiana Z, Elisaf M, et al. Potential aetiological factors concerning the development of osteonecrosis of the femoral head. Eur J Clin Invest. 2000;30(3):215-221.