Mitochondria membrane potentials and reactive oxygen species of vascular endothelial cells during senescence process

doi:10.3969/j.issn.2095-4344.2013.15.010

Abstract

Abstract:

BACKGROUND: Balance among aging, apoptosis and regeneration of vascular endothelial cells exerts a critical role in maintenance of the function of normal vessels. Mitochondria are a key cell organelle in the body, which cannot only support energy for the cells via adenosine triphosphate synthesis, but also control the procedure death, aging of cells as well as many pathophysical metabolism processes.
OBJECTIVE: To investigate the relationship between mitochondria membrane potentials and reactive oxygen species of endothelial cells during growing and senescence process in order to explore the dysfunction occurring during the senescence process.
METHODS: Human umbilical vein endothelial cells were cultured in vitro and passaged. Passages 2, 4, 6 and 8 human umbilical vein endothelial cells were selected to detect those mitochondria membrane potentials and reactive oxygen species. Levels of reactive oxygen species and mitochondria membrane potentials were checked by flow cytometry. At the same time, passage 2 and 8 cells were selected for observation of normal and aging ultramicro-morphoses by electron microscope.
RESULTS AND CONCLUSION: During the passage and senescence, mitochondria membrane potentials were decreased gradually with cell passage, while the levels of reactive oxygen species were increased firstly and then decreased. The number of mitochondria and reticulum was reduced along with cell passage. In replicate aging process, the mitochondrial membrane potential was decreased and the mitochondrion was damaged. At the first time the production of reactive oxygen species was increased, while decreased along with the cell degeneration.

Key words: tissue construction, vascular tissue construction, endothelium, aging, mitochondria, reactive oxygen species, membrane potential, cells, passage, vessels, advanced micro devices, degeneration

CLC Number:

R318

Zhao Hai-mei, Yang Bin, Cheng Bei. Mitochondria membrane potentials and reactive oxygen species of vascular endothelial cells during senescence process[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.15.010.

Figures/Tables 3

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