Bone marrow mesenchymal stem cells cultured in artificial meninges repair infarcted myocardium in rats

doi:10.3969/j.issn.2095-4344.2013.14.012

Abstract

Abstract:

BACKGROUND: Stem cell transplantation has broad application prospects in the treatment of myocardial infarction. To find the ideal cell type and the effective transplantation method is the key factor to improve.
OBJECTIVE: To investigate the effect and safety of artificial meninges combined with bone marrow mesenchymal stem cells on repairing infarcted heart.
METHODS: Bone marrow mesenchymal stem cells were obtained by whole bone marrow culture method, and subcultured to the third generation. Then bone marrow mesenchymal stem cells were labeled with 2-(4-amidinophenyl)- 6-indolecarbamidine dihydrochloride and seeded on the cells and artificial meningeal complex. Sprague Dawley rats model of myocardial infarction were established, and 60 rats were randomly divided into sham-operation group, myocardial infarction group, artificial meningeal group, and cells and artificial meningeal complex group. The cardiac function parameters were tested at 4 weeks after transplantation, Western blot was used to test the expression of myocardial tissue connexin 43, and the survival rate was calculated after myocardial infarction.
RESULTS AND CONCLUSION: Frozen sections of the heart tissues showed a few blue-stained cells under the fluoroscope in the complex group at 4 weeks after myocardial infarction and transplantation, which indicated that a few bone marrow mesenchymal stem cells survived in the heart. Compared with myocardial infarction group and artificial meningeal group, left ventricular function was improved, the expression of connexin 43 protein and the survival rate were increased in the complex group (P < 0.05). Artificial meninges combined with bone marrow mesenchymal stem cells can improve cardiac function and survival rate in myocardial infarction rats.

Key words: stem cells, stem cell transplantation, myocardial infarction, artificial meninges, bone marrow mesenchymal stem cells, myocardial regeneration, biological tissue engineering, stem cell photographs- containing paper

CLC Number:

R394.2

Ma Hong-fen, Zhang Xiao-gang, Shi Ruo-fei, Xiong Ting-lin, Zhao Xia. Bone marrow mesenchymal stem cells cultured in artificial meninges repair infarcted myocardium in rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2552-2557.

Figures/Tables 5

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