In vivo MRI-traced bone marrow mesenchymal stem cell transplantation in
myocardial infarction rats

doi:10.3969/j.issn.2095-4344.2013.14.001

Abstract

Abstract:

BACKGROUND: The continuous monitoring of the in vivo survival, distribution, migration, proliferation and differentiation of the stem cells is important to evaluate the efficacy and safety of stem cells transplantation.
OBJECTIVE: To observe the distribution and migration of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells in ischemic myocardial tissue traced with MRI.
METHODS: Rat bone marrow mesenchymal stem cells were separated and cultured by direct attachment method. The surface antigens of the bone marrow mesenchymal stem cells were identified. The bone marrow mesenchymal stem cells were labeled by the novel superparamagnetic iron oxide. The feasibility of labeled bone marrow mesenchymal stem cells was determined by MRI in vitro. Trypan blue exclusion test and methylthiazolyldphenyl-tetrazolium bromide colorimetric test were performed to detect the activity and proliferation of the labeled cells. A total of 60 Sprague Dawley rats were divided into three groups, and the rats were used to establish the myocardial infarction model. At 2 weeks after modeling, the rats were re-implanted with phosphate-buffered solution containing labeled bone marrow mesenchymal stem cells, phosphate-buffered solution containing unlabeled bone marrow mesenchymal stem cells and phosphate-buffered solution in the same dose, respectively. All rats in each group underwent MRI at 1 day and 3 weeks after transplantation to dynamically monitor the distribution and migration of transplanted cells. CD90 immunohistochemistry was done according to MRI.
RESULTS AND CONCLUSION: After superparamagnetic iron oxide labeling, the Prussian blue staining showed that the blue iron particles were inside the cytoplasm and the labeling rate was 99%. There was no statistically difference of trypan blue exclusion rate and methylthiazolyldphenyl-tetrazolium bromide absorption value between labeled and unlabeled cells. Labeled cells could be detected as low signal intensity on T2WI and T2W/FFE with in vitro MRI. Labeled bone marrow mesenchymal stem cells showed round low signal intensity on T2WI, T2W/FFE at the edge of the infarcted myocardium at 1 day after transplantation, and the initial low signal became obscure gradually, extended and contrast reduced at 3 weeks after transplantation. CD90 immunohistochemistry staining confirmed that transplanted bone marrow mesenchymal stem cells could migrate from the border to the infarcted region. It was feasible to label the bone marrow mesenchymal stem cells with the novel superparamagnetic iron oxide in rats and the labeled bone marrow mesenchymal stem cells could be tracked on MRI in vitro.

Key words: stem cells, bone marrow-derived stem cells, magnetic resonance imaging, myocardial infarction, bone marrow mesenchymal stem cells, stem cell transplantation, in vivo, tracer, rats, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Hua Ping, Wang You-yu, Yang Song-ran, Liu Jia-liang, Liu Li-bao, Tao Jun, Yang Yan-qi. In vivo MRI-traced bone marrow mesenchymal stem cell transplantation in
myocardial infarction rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2471-2479.

Figures/Tables 13

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