Trypsin and dexamethasone affect proliferation and cytoskeleton protein expression of human lung fibroblasts

doi:10.3969/j.issn.2095-4344.2013.11.018

Abstract

Abstract:

BACKGROUND: In addition to the supporting function, lung fibroblasts play a pivotal role in the process of lung tissue injury and repair through proliferation, contraction, chemotaxis and secretion of the extracellular matrix.
OBJECTIVE: To investigate the effects of trypsin and dexamethasone on the proliferation and cytoskeleton protein expression of lung fibroblasts.
METHODS: Human lung fibroblasts were isolated and cultured in vitro followed by trypsin (0, 0.5, 1.0, 5, 10 mg/L) treatment for 24 hours or dexamethasone (10^-9-10^-6 mol/L) treatment for 72 hours. The expression of vimentin and α-smooth muscle actin was detected by immunoblotting. 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay was utilized for measuring fibroblasts proliferation.
RESULTS AND CONCLUSION: Trypsin at lower concentrations (0.1-0.5 mg/L) significantly stimulated lung fibroblasts proliferation, while higher concentrations (1-10 mg/L) of trypsin inhibited lung fibroblasts proliferation. No effect on fibroblasts proliferation was observed with dexamethasone. Trypsin significantly increased the expression of α-smooth muscle actin in a dose-dependent manner, but had no influence on vimentin expression. Dexamethasone (10^-9-10^-6 mol/L) significantly inhibited vimentin expression in a concentration-dependent manner. The data reveal that trypsin and glucocorticosteroids may participate in the tissue remodeling process of chronic inflammatory airway diseases through affecting lung fibroblasts proliferation and the expression of cytoskeleton protein.

Key words: tissue construction, cytology experiment in tissue construction, trypsin, glucocorticoids, lung fibroblasts, vimentin, actin, immunoblotting, dexamethasone, cytoskeletal proteins, fibroblast proliferation, chronic obstructive pulmonary disease, the National Natural Science Foundation of China, tissue construction photographs-containing paper

CLC Number:

R318

Fang Qiu-hong, Shui Chao-xiang, Wang Yao-yao, Wang Rui-qin, Wang Jing, Ma Ying-min. Trypsin and dexamethasone affect proliferation and cytoskeleton protein expression of human lung fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 2020-2025.

Figures/Tables 6

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