Auto-adipose stem cells facilitate autologous fat transfer for breast augmentation

doi:10.3969/j.issn.2095-4344.2013.05.018

Abstract

Abstract:

BACKGROUND: Human adipose stem cells have the capability of multipotential differentiation and have a promising prospect in the field of tissue engineering and regenerative medicine.
OBJECTIVE: To evaluate the technology and efficacy of human adipose stem cells in autologous fat grafting breast augmentation.
METHODS: Fifteen patients with auto-adipose injection breast augmentation were followed-up for 3-6 months. The whole operation was separated into two phases, in the first phase, 15-20 mL adipose tissue was aspirated and digested with collagenase Ⅰ to separate and amplify the human adipose stem cells. When cultured to passage 2, the plastic adherent stem cells were up to 1×108 and verificated by immunophenotype, differentiation and genetic stability assays before transplantation. In the second phase, 300-450 mL adipose tissue was aspirated and transplanted combined with human adipose stem cells.
RESULTS AND CONCLUSION: The passage 2 human adipose stem cells exhibited typical mesenchymal stem cells characteristics and maintained genetic stability. The 3-6 months follow-up demonstrated that there was no apparent adipose absorption, infection, pain and upper limb activity limitation, and the effect was good. The authors suggested that autologous adipose derived stem cells-mediated auto-adipose injection breast augmentation is a practical and efficacious method.

Key words: organ transplantation, tissue transplantation, tissue and cell transplantation, adipose derived stem cells, mesenchymal stem cells, genetic stability, immunophenotype, differentiation potential, breast augmentation, auto-adipose injection, photographs-containing paper of organ transplantation

CLC Number:

R318

Yan Jun-ling, Di Guo-hu, Ding Hong, Zhang Wei, Liu Jia-qi, Tang Su-yang. Auto-adipose stem cells facilitate autologous fat transfer for breast augmentation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(5): 878-885.

Figures/Tables 10

References

[1] Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu xueshubu. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2010;14(16):3006-3006. 《中国组织工程研究与临床康复》学术部.关于纳米材料与纳米技术的学术争鸣：本刊学术部[J].中国组织工程研究与临床康复,2010,14(16):3006-3006.

[2] Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu xueshubu. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009; 13(34):6622-6623. 《中国组织工程研究与临床康复》学术部.让昨天告诉今天：隆胸假体材料学术与技术发展的足迹[J]. 中国组织工程研究与临床康复,2009,13(34):6622-6623.

[3] Wang M, Wang XY, Hou XD, et al. ZHongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2010;14(8): 1488-1490. 王慕,王晓阳,侯晓冬,等. 聚丙烯酰胺水凝胶注射后伴发乳房恶性肿瘤:2例报道[J]. 中国组织工程研究与临床康复,2010, 14(8): 1488-1490.

[4] Shen ZL, Jia WX, Cai YX, et al. Zuzhi Gongcheng yu Chongjian Waike Zazhi. 2007;3(3):154-156. 沈尊理,贾万新,蔡嬿娴,等.聚丙烯酰胺水凝胶注射隆乳术后并发症处理临床分析[J].组织工程与重建外科杂志,2007,3(3): 154-156.

[5] Ding KX, Dong P, Yang YP, et al. Zhongguo Huazhuangpin. 2012;13:54-64. 丁克祥,董萍,杨永鹏,等.整形美容中隆胸材料和丰胸制剂及脂肪细胞相关基因的应用与研究进展[J]. 中国化妆品,2012,13: 54-64.

[6] Gerlach JC, Over P, Turner ME, et al. Perivascular Mesenchymal Progenitors in Human Fetal and Adult Liver. Stem Cells Dev. 2012.

[7] Illouz YG. The fat cell "graft": a new technique to fill depressions. Plast Reconstr Surg. 1986;78(1):122-123.

[8] Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007;100(9):1249-1260.

[9] Kadam S, Govindasamy V, Bhonde R. Generation of functional islets from human umbilical cord and placenta derived mesenchymal stem cells. Methods Mol Biol. 2012; 879:291-313.

[10] Zhang WZ, Li GQ, Chen YQ,et al. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(40): 7833-7837. 张卫泽,李国庆,陈永清,等.人脂肪间充质干细胞的分离培养及其遗传稳定性[J].中国组织工程研究与临床康复,2009,13(40): 7833-7837.

[11] Anderson DJ,Gage FH,Weissman IL,et al.Can stem cells cross lineage boundaries .Nat Med. 2001;7(4):393-395.

[12] Zuk PA,Zhu M,Ashjian P,et al. Human adipose tissue is a source of multipotent stem cells.Mol Biol Cell.2002;13(12): 4279-4295.

[13] Zhu Y, Liu T, Song K, et al. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct. 2008;26(6): 664-675.

[14] Lee JH, Kemp DM. Human adipose-derived stem cells display myogenic potential and perturbed function in hypoxic conditions. Biochem Biophys Res Commun. 2006;341(3): 882-888.

[15] Choi YS, Vincent LG, Lee AR, et al. Mechanical derivation of functional myotubes from adipose-derived stem cells. Biomaterials. 2012;33(8):2482-2491.

[16] Lee JH, Kosinski PA, Kemp DM. Contribution of human bone marrow stem cells to individual skeletal myotubes followed by myogenic gene activation. Exp Cell Res. 2005;307(1): 174-182.

[17] Banas A, Teratani T, Yamamoto Y, et al. Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes. Hepatology. 2007;46(1):219-228.

[18] Banas A. Purification of adipose tissue mesenchymal stem cells and differentiation toward hepatic-like cells. Methods Mol Biol. 2012;826:61-72.

[19] Xiu ZF, Huang HP, et al. ZHonghua Zhengxing Waike Zazhi. 2001;17(5):289-289. 修志夫,黄恒平. 脂肪颗粒注射降乳并发硬结的病理观察及处理[J]. 中华整形外科杂志,2001,17(5):289-289.

[20] Sun Z, Jiang AM. Yixue Zongshu. 2012;18(15):2401-2403. 孙哲,蒋爱梅. 脂肪干细胞与碱性成纤维细胞生长因子在颗粒脂肪移植中应用的研究进展[J]. 医学综述,2012,18(15):2401-2403.

[21] Xu GQ, Wang BJ, Zhang YH, et al. Zhongguo Meirong Yixue. 2012;21(7):1111-1112. 徐刚强,王保健,张跃辉,等. Body—Jet水动力系统应用于自体脂肪移植隆胸的临床体会[J]. 中国美容医学,2012,21(7): 1111-1112.

[22] Liu NJ, Guan YP, Wang Y, et al. ZHongguo Meirong Yixue. 2011,;20(7):1178-1180. 刘乃军,管延萍,王艳,等.湿性脂肪干细胞辅助自体脂肪移植术[J].中国美容医学,2011,20(7):1178-1180.

[23] Zhu Y, Liu T, Song K, et al. ADSCs differentiated into cardiomyocytes in cardiac microenvironment. Mol Cell Biochem. 2009;324(1-2):117-129.

[24] Vija L, Farge D, Gautier JF, et al. Mesenchymal stem cells: Stem cell therapy perspectives for type 1 diabetes. Diabetes Metab. 2009;35(2):85-93.

[25] Fändrich F, Ungefroren H. Customized cell-based treatment options to combat autoimmunity and restore beta-cell function in type 1 diabetes mellitus: current protocols and future perspectives. Adv Exp Med Biol. 2010;654:641-665.

[26] Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg. 2008;32(1):48-57.

[27] Yoshimura K, Asano Y, Aoi N, et al. Progenitor-enriched adipose tissue transplantation as rescue for breast implant complications. Breast J. 2010;16(2):169-175.

[28] Zhu SW, Song GB, Xu XW, et al. Zhongguo Meirong Yixue. 2012;21(1):78-81. 祝顺武,宋广滨,徐学武,等. 脂肪注射移植后皮肤质地改善的研究[J]. 中国美容医学,2012,21(1):78-81.

[29] Ma WH, Han YZ, Sang J, et al. Linchuang yu Shiyan Binglixue Zazhi. 2012;28(1):19-21. 马文浩,韩玉贞,桑晶,等. BRCA1在散发性乳腺癌干细胞中的表达及意义[J]. 临床与实验病理学杂志,2012,28(1):19-21.

[30] Matsumoto D,Sato K,Ganda K,et al. Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection.Tissue Eng.2006;12(12): 3375-3382.