Whether the cells of different lumbar discs can produce interleukin-1 beta

doi:10.3969/j.issn.2095-4344.2013.04.001

Abstract

Abstract:

BACKGROUND: The studies found that there are cytokines and inflammatory cytokines in the degenerative intervertebral disc, but it is still unknown that whether they come from the outer intervertebral disc or inner intervertebral disc.
OBJECTIVE: To investigate whether the cells of different lumbar discs can produce inflammatory cytokine interleukin-1β.
METHODS: The cells of rabbit lumbar disc were isolated and cultured and then identified. The cells were induced with Fn120 fibronectin fragments, and PBS was used in the control group. The expression of interleukin-1β was detected by immunofluorescence method and reverse transcription-PCR.
RESULTS AND CONCLUSION: In the experimental group, the cartilage cells of intervertebral disc and fibroblasts of annulus fibrosis could produce the interleukin-1β. The results showed that cartilage endplate cells and the fibroblasts of annulus fibrosis can produce interleukin-1β.

Key words: bone and joint implants, spinal implants, intervertebral disc cells, disc degeneration, interleukin-1β, fibronectin, endplate cells, fibroblasts of annulus fibrosis, autocrine, cell culture, cytokine, inflammatory mediators, other grants-supported paper, photographs-containing paper of bone and joint implants

CLC Number:

R318

Hu Bao-shan, Xu Xi-jun, Guo Yuan-li, Rui Gang. Whether the cells of different lumbar discs can produce interleukin-1 beta[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(4): 571-575.

Figures/Tables 4

References

[1] Takahashi H, Suguro T, Okazima Y, et al. Inflammatory cytokines in the herniated disc of the lumbar spine. Spine. 1996;21(2):218-224.

[2] Wang J,Markova D,Anderson DG,et al. TNF-α and IL-1β promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc. J Biol Chem. 2011;286(46):39738-39749.

[3] de Souza Grava AL, Ferrari LF, Defino HL. Cytokine inhibition and time-related influence of inflammatory stimuli on the hyperalgesia induced by the nucleus pulposus. Eur Spine J. 2012;21(3):537-545.

[4] Cuellar JM, Montesano PX, Carstens E. Role of TNF-alpha in sensitization of nociceptive dorsal horn neurons induced by application of nucleus pulposus to L5 dorsal root ganglion in rats. Pain. 2004;110(3):578-587.

[5] Kim SJ, Park SM, Cho YW, et al. Changes in expression of mRNA for interleukin-8 and effects of interleukin-8 receptor inhibitor in the spinal dorsal horn in a rat model of lumbar disc herniation. Spine (Phila Pa 1976). 2011;36(25):2139-2146.

[6] Studer RK, Vo N, Sowa G, et al. Human nucleus pulposus cells react to IL-6: independent actions and amplification of response to IL-1 and TNF-α.Spine. 2011;36(8):593-599.

[7] Melrose J,Shu C,Young C, et al. Mechanical destabilization induced by controlled annular incision of the intervertebral disc dysregulates metalloproteinase expression and induces disc degeneration. Spine (Phila Pa 1976). 2012;37(1):18-25.

[8] Melrose J, Shu C, Young C,et al.Mechanical destabilisation induced by controlled annular incision of the intervertebral disc dysregulates metalloproteinase expression and induces disc degeneration. Spine (Phila Pa 1976). 2011.

[9] Yasuoka H, Asazuma T, Nakanishi K, et al. Effects of reloading after simulated microgravity on proteoglycan metabolism in the nucleus pulposus and anulus fibrosus of the lumbar intervertebral disc: an experimental study using a rat tail suspension model. Spine (Phila Pa 1976). 2007;32(25): E734-40.

[10] Mwale F, Masuda K, Pichika R, et al. The efficacy of Link N as a mediator of repair in a rabbit model of intervertebral disc degeneration. Arthritis Res Ther. 2011;13(4):R120.

[11] Chun HJ, Kim YS, Kim BK, et al. Transplantation of Human Adipose-Derived Stem Cells in a Rabbit Model of Traumatic Degeneration of Lumbar Discs. World Neurosurg. 2011.

[12] Benz K,Stippich C,Fischer L,et al.Intervertebral disc cell- and hydrogel-supported and spontaneous intervertebral disc repair in nucleotomized sheep. Eur Spine J. 2012.

[13] Park JO, Lee BH, Kang YM, et al. Inflammatory Cytokines Induce Fibrosis and Ossification of Human Ligamentum Flavum Cells. J Spinal Disord Tech. 2012.

[14] Bu G, Hou S, Ren D, et al. Increased Expression of Netrin-1 and its Deleted in Colorectal Cancer Receptor in Human Diseased Lumbar Intervertebral Disc Compared with Autopsy Control. Spine (Phila Pa 1976). 2012. [Epub ahead of print]

[15] Taher F, Essig D, Lebl DR, et al. Lumbar degenerative disc disease: current and future concepts of diagnosis and management. Adv Orthop. 2012;2012:970752.

[16] Yurube T, Takada T, Suzuki T, et al. Rat tail static compression model mimics extracellular matrix metabolic imbalances of matrix metalloproteinases, aggrecanases, and tissue inhibitors of metalloproteinases in intervertebral disc degeneration. Arthritis Res Ther. 2012;14(2):R51.