Notch signal pathway effect on receptor protein regulation by transforming growth factor beta in primary human keratinocytes

doi:10.3969/j.issn.2095-4344.2013.02.020

Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (2): 296-300.doi: 10.3969/j.issn.2095-4344.2013.02.020

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Notch signal pathway effect on receptor protein regulation by transforming growth factor beta in primary human keratinocytes

Xue Si-liang¹, Wang Xiao-shan², Li Dong-chuan³, Zhang Jue^{4, 5}

1 Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
2 Department of Oncology, Sichuan Provincial People’s Hospital, Chengdu 610072, Sichuan Province, China
3 Department of Obstetrics and Gynecology, the 363 Hospital of Chengdu City, Chengdu 610041, Sichuan Province, China
4 Zunyi Medical and Pharmaceutical College, Zunyi 563000, Guizhou Province, China
5 Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

Received:2012-04-13 Revised:2012-05-21 Online:2013-01-08 Published:2013-01-08
Contact: Zhang Jue, Master, Lecturer, Zunyi Medical and Pharmaceutical College, Zunyi 563000, Guizhou Province, China; Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China zhangjue25@163.com
About author:Xue Si-liang☆, Doctor, Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China xuesiliang@163.com

Abstract

Abstract:

BACKGROUND: Regulation of the receptor protein tyrosine phosphatase kappa (RPTP-κ) in the skin is essential, while transforming growth factor beta (TGF-β) appears to be an upstream factor of its regulation. As Notch signaling pathway is similar to TGF-β signaling pathway, whether Notch participates in the regulation of RPTP-κ transcription by TGF-β signaling?
OBJECTIVE: To find out the role of Notch signal in regulation of RPTP-κ transcription by TGF-β.
METHODS: Jagged-1 and gamma-secretase inhibitors (GSI) were use respectively to activate and inhibit Notch signal followed by addition of TGF-β. Simultaneously, control group was set. Real-time PCR was used to determine RPTP-k mRNA expression in human keratinocytes.
RESULTS AND CONCLUSION: After adding TGF-β into 40% confluent keratinocytes, the RPTP-κ mRNA expression was higher at different time as compared with the control group. Following Jagged-1 activated Notch signaling pathway, the mRNA expression of RPTP-κ in the keratinocytes was higher when Jagged-1, TGF-β or their combination was added into the cells as compared with the control group (P < 0.05, P < 0.01). Following GSI inhibited Notch signaling pathway, the mRNA expression of RPTP-κ in the keratinocytes was higher only when TGF-β was added into the cells as compared with the control group (P < 0.01), and no significant difference was seen when GSI alone or combination of TGF-β and GSI was added into the cells as compared with the control group (P > 0.05). These findings indicate that the mRNA expression of RPTP-κ in the keratinocytes was increased after TGF-β was added, and following activation or inhibition of Notch signaling, the mRNA expression of RPTP-κ was significantly increased or suppressed. Therefore, Notch signaling is very important and indispensable in the regulation of RPTP-κ by TGF-β.

Key words: tissue construction, cytological experiments of tissue construction, transforming growth factor beta, receptor protein tyrosine phosphatase kappa, human keratinocytes, Notch, NICD

CLC Number:

R318

Xue Si-liang, Wang Xiao-shan, Li Dong-chuan, Zhang Jue. Notch signal pathway effect on receptor protein regulation by transforming growth factor beta in primary human keratinocytes[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 296-300.

Figures/Tables 3

References

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Notch signal pathway effect on receptor protein regulation by transforming growth factor beta in primary human keratinocytes

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