Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8476-8480.doi: 10.3969/j.issn.2095-4344.2012.45.021

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Connexin-43 expression in cardiac tissue after adipose-derived stem cells transplantation for treatment of acute myocardial infarction

Jie Ling-jun1, Wang Yi-qing1,2, Ma Qun-cao2, Chen Xiao-min2, Luo Hong2, Zhang Cheng-Wei1   

  1. 1Xiehe Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China
    2Department of Cardiology, Xiamen Heart Centre, Xiamen 361004, Fujian Province, China
  • Received:2012-04-05 Revised:2012-04-24 Online:2012-11-04 Published:2012-11-04
  • Contact: Wang Yi-qing, M.D., Chief physician, Professor, Xiehe Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China; Department of Cardiology, Xiamen Heart Centre, Xiamen 361004, Fujian Province, China wang_gina@163.com
  • About author:Jie Ling-jun★, Studying for master’s degree, Xiehe Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China jielingjun11@163.com

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Abstract:

BACKGROUND: The therapeutic effect of adipose-derived stem cells (ADSC) as a novel kind of seed cells on myocardial infarction in rats has been rarely reported.
OBJECTIVE: To investigate the effects of ADSCs transplantation on cardiac function and connexin-43 expression in rats with acute myocardial infarction.
METHODS: Rat ADSCs were isolated and cultured in vitro. Twenty-four Sprague-Dawley rats were divided into sham-operated, myocardial infarction and ADSCs groups. Rats in the former two groups were prepared into acute myocardial infarction models by ligation of the left anterior descending branch. In the sham-operated group, only chest was cut open without ligation. In the ADSCs group, at 30 minutes after myocardial infarction, ADSCs were administered via four points, 25 μL per point. Rats in the sham-operated and myocardial infarction groups received identical amounts of phosphate buffered saline. At 2 weeks after ADSCs transplantation, index examination was performed.
RESULTS AND CONCLUSION: Echocardiogram showed that left ventricular ejection fraction, and left ventricular fractional shortening were significantly greater in the myocardial infarction group than in the ADSCs group (P < 0.05). Masson’s trichrome staining demonstrated that fibrinous exudate level was significantly lower in the ADSCs group than in the myocardial infarction group (P < 0.05). Immunofluorescent results showed that connexin-43 expression and vascular density in the ADSCs group were significantly increased than in the myocardial infarction group (P < 0.05-0.01). Real-time PCR showed that connexin-43 mRNA expression in the ADSCs group was significantly greater than in the myocardial infarction group (P < 0.01). ADSCs transplantation can greatly improve the cardiac function, reduce fibrinous exudates and up-regulate connexin-43 expression after myocardial infarction.

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