Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7688-7693.doi: 10.3969/j.issn.2095-4344.2012.41.018

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Effect of stromal cell derived factor-1 on migration of bone marrow mesenchymal stem cells and bone healing

Chen Wei1, Chen Jian-mei2, Zhang Xian-yu3, Yao Xiao-dong2, Xu Hao2   

  1. 1Department of Orthopedics, Fuzhou Second Hospital, Fuzhou 350007, Fujian Province, China
    2Department of Orthopedics, Nanjing General Hospital of Fuzhou Military Command, Fuzhou 350025, Fujian Province, China
    3Department of Orthopedics, Shangrao People's Hospital of Jiangxi Province, Shangrao 334000, Jiangxi Province, China
  • Received:2012-07-02 Revised:2012-08-09 Online:2012-10-07 Published:2012-10-07
  • Contact: Xu Hao, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Nanjing General Hospital of Fuzhou Military Command, Fuzhou 350025, Fujian Province, China xiuhao@medmail.com.cn
  • About author:Chen Wei★, Master, Attending physician, Department of Orthopedics, Fuzhou Second Hospital, Fuzhou 350007, Fujian Province, China 76202846@qq.com

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Abstract:

BACKGROUND: The number and function of bone marrow mesenchymal stem cells in the fracture end are closely correlated with fracture healing. Stromal cell derived factor-1 can promote the chemotactic functions of bone marrow msenchymal stem cells.
OBJECTIVE: To establish the left tibia fracture model by using the chimeric mice with stably expressing green fluorescence protein positive bone marrow mesenchymal stem cells, in order to evaluate the protective effect of stromal cell derived factor-1 on the migration of bone marrow mesenchymal stem cells and bone healing, and to explore the mechanism.
METHODS: Green fluorescence protein positive bone marrow mesenchymal stem cells were harvested from bone marrow of green fluorescence protein transgenic C57BL mice by density gradient centrifugation method. The green fluorescence protein transgenic bone marrow mesenchymal stem cells combined with the non-adherent bone marrow cells in the male mice were transplanted after X-ray irradiation for establishing the chimeric mice model. After chimeric mice were established, left tibia fracture was made and induced with stromal cell derived factor-1 and stromal cell derived factor-1 antibody, and the control group was set.
RESULTS AND CONCLUSION: The amount of bone marrow mesenchymal stem cells in stromal cell derived factor-1 group was higher than that in the control group and anti-stromal cell derived factor-1 group at 1, 3, 7 and 14 days after modeling (P < 0.05); the sum of callus in stromal cell derived factor-1 group were greater than that in the control group and anti-stromal cell derived factor-1 group at 14 and 21 days after modeling (P < 0.05); the average peak deformation of the fracture-healing site in the stromal cell derived factor-1 group at 28 days after modeling was greater than that in the control group and anti-stromal cell derived factor-1 group (P < 0.05); all the indicators above in the control group were greater than those in the anti-stromal cell derived factor-1 group. At 28 days after modeling in the stromal cell derived factor-1 group, the sum of callus was decreased (P < 0.05), the trabecular was integrated into the film and part of the marrow cavity was recanalized. The medullary cavity in the control group and anti-stromal cell derived factor-1 group was not recanalized. Stromal cell derived factor-1 can promote bone marrow mesenchymal stem cells to migrate into the fracture site and can accelerate fracture healing.

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