Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7694-7697.doi: 10.3969/j.issn.2095-4344.2012.41.019

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Brain-derived neurotrophic factor promotes the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cells

Tang Yuan-yuan1,2, Li Zhen-yu3, Yin Yan-yan4, Ji Li-li2, Wang Zhen-yu2   

  1. 1Department of Histology and Embryology, College of Basic Medical Sciences, 3School of Basic Medicine Sciences, 4School of Pharmaceutical Sciences, Xinxiang Medical University, Xinxiang 453000, Henan Province, China; 2Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China
  • Received:2012-02-28 Revised:2012-04-08 Online:2012-10-07 Published:2012-10-07
  • Contact: Wang Zhen-yu, Master, Professor, Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China zywang60@163.com
  • About author:Tang Yuan-yuan★, Master, Teaching assistant, Department of Histology and Embryology, College of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453000, Henan Province, China; Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China tangyuanyuan@xxmu.edu.cn

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Abstract:

BACKGROUND: There is evidence that brain-derived neurotrophic factor can maintain neuronal survival, influence neuronal migration, and promote the survival and differentiation of neural stem cells in vitro.
OBJECTIVE: To investigate the effects of brain-derived neurotrophic factor on the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cells.
METHODS: Neonatal 1-day hypoglycemia rat hippocampal tissue was harvested and subjected to primary culture, subculture and cell monoclonal culture. Then cells were divided into two parts: one part was used for identification of neural stem cells, and the other part was divided into three groups as per different concentrations of brain-derived neurotrophic factors (0, 100, 200 μg/L). Passage 4 cells were used for induced differentiation. Neuron-specific enolase immunofluorescence staining was used for neuronal identification and positive cells were counted.
RESULTS AND CONCLUSION: After monoclonal culture, three groups of cells were positive for nestin. After induced differentiation, cells were positive for neuron-specific enolase and glial fibrillary acidic protein staining. Neural stem cells in the 100 μg/L and 200 μg/L groups grew faster, and produced higher proportion of neuron-specific enolase-positive cells than those in the 0 μg/L group (P < 0.05). But there was no significant difference between 100 μg/L group and 200 μg/L group. These findings suggest that brain-derived neurotrophic factor promotes the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cells.

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