Abstract:

BACKGROUND: The method that using chemokine or chemotactic polypeptide to recruit immune effector cells to converge in tumor cells to arise anti-tumor immunity is expected to become a new strategy for tumor treatment.
OBJECTIVE: To discuss the effects of chemokine expression on the invasion and metastasis in the ovarian tumor stem cells for giving new ideas of ovarian cancer research.
METHODS: Expression of CXCR4 in ovarian cancer cell line SW626 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Transwell room was used to detect the effects of CXCL12 and CXCL12/CXCR4 on chemotaxic invasion and metastasis of SW626 cells.
RESULTS AND CONCLUSION: RT-PCR confirmed that there was CXCR4 expression in the SW626 cell lines, but no expression was found in the Anglne cells. CXCR4 expression could increase the number of ovarian tumor stem cells subjected to chemotactic invasion and metastasis, while CXCR4 addition could inhibit above-mentioned effects. CXCR12, a chemotatic factor of ovarian tumor stem cells, can promote the chemotaxic activity and invasion and metastasis activity mediated by CXCR4 receptor.