Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7673-7676.doi: 10.3969/j.issn.2095-4344.2012.41.015

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Induction of transplantation tolerance to fully mismatched skin allografts in mice by syngeneic hematopoietic stem cells

Xu San-rong, Zhou Qing, Han Bo, Liu Xia, Wu Wei-jiang, Xu Hui-ling   

  1. Second Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
  • Received:2012-01-21 Revised:2012-03-09 Online:2012-10-07 Published:2012-10-07
  • About author:Xu San-rong☆, M.D., Chief physician, Second Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China zjzouking@sina.com

Abstract:

BACKGROUND: The optimal effect of organ transplantation tolerance is to induce the specific immunologic tolerance to transplantation antigens.
OBJECTIVE: To study the possibility of using syngeneic hematopoietic stem cells to reconstitute immune system and induce transplantation tolerance to fully mismatched skin allografts in mice.
METHODS: BALB/c mouse marrow was taken. Syngeneic skin transplantation was performed taking C57BL/6 mice as donors and BALB/c mice as recipients. Thirty-two BALB/c mice were randomly divided into four groups: control, cyclosporin A, cyclosporin A + total body irradiation, cyclosporin A + total body irradiation, cyclosporin A + total body irradiation + bone marrow transplantation.
RESULTS AND CONCLUSION: In the cyclosporin A + total body irradiation group, all mice died within 10 days and the counts of peripheral blood white blood cells were persistently declined. In the cyclosporin A + total body irradiation + bone marrow transplantation group, mice survived during the experimentation, the counts of peripheral blood white blood cells decreased to the bottom after 6 days of irradiation and then rebounded, and increased to the level of the cyclosporine A group after 21 days of irradiation (P > 0.05). The average survival time of skin grafts in the cyclosporin A + total body irradiation + bone marrow transplantation group was significantly longer than that in the other groups (P < 0.01). There were less infiltrated cells and less destructed tissue, and decreased proliferation of recipient mouse spleen cells in the cyclosporin A + total body irradiation + bone marrow transplantation group than in the other groups. These findings suggest that immune reconstitution with syngeneic bone marrow cells can obviously prolong the survival time of mouse skin allografts and induce donor-specific tolerance.

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