Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7617-7620.doi: 10.3969/j.issn.2095-4344.2012.41.004

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Effects of various volume fractions of umbilical cord serum on the proliferation ability of human bone marrow mesenchymal stem cells

Lei Xiao-yu, Zhou Xin-Fu, Luo Zi-mian, Liu Li-hua, Liu Kang   

  1. Department of Hematology of Xiangtan Central Hospital & Xiangtan Clinical College of Central South University, Xiangtan 411100, Hunan Province, China
  • Received:2012-01-12 Revised:2012-02-14 Online:2012-10-07 Published:2012-10-07
  • Contact: Zhou Xin-fu, M.D., Chief physician, Department of Hematology of Xiangtan Central Hospital & Xiangtan Clinical College of Central South University, Xiangtan 411100, Hunan Province, China zhouxfhoom@ yahoo.com.cn
  • About author:Lei Xiao-yu★, Studying for master’s degree, Attending physician, Department of Hematology of Xiangtan Central Hospital & Xiangtan Clinical College of Central South University, Xiangtan 411100, Hunan Province, China Daisy8008@gmail.com

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Abstract:

BACKGROUND: Culture of bone marrow mesenchymal stem cells (BMSCs) after preparation of umbilical cord serum has become an increasing research area. However, there have been no reports regarding the effects of different volume fractions of umbilical cord serum on the proliferation ability of BMSCs.
OBJECTIVE: To compare the effects of different volume fractions of umbilical cord serum on the proliferation ability of BMSCs.
METHODS: Twenty bone marrow samples were obtained under aseptic conditions. The mononuclear cells were extracted by density gradient centrifugation method, and cultured with 5%, 7.5%, 10%, 15%, 20% umbilical cord serum + DMEM/F12 culture medium respectively. Passage 3 BMSCs were included in this study. MTT assay was used to investigate the effects of different volume fractions of umbilical cord serum on the proliferation ability of BMSCs.
RESULTS AND CONCLUSION: Compared with the 15%, 20% volume fractions of umbilical cord serum + DMEM/F12 medium groups, the proliferation ability of BMSCs was significantly stronger than in the 5%, 7.5%, 10% volume fractions of umbilical cord serum + DMEM/F12 medium groups (P < 0.05). These findings suggest that the proliferation ability of BMSCs is more active at low volume fractions of human umbilical cord serum culture system.

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