Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7612-7616.doi: 10.3969/j.issn.2095-4344.2012.41.003

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Effects of cadmium chloride on micronucleus rate and chromosome aberration rate in mouse bone marrow mesenchymal stem cells

Liu Yong-qi1,2, Da Rui1, Dou Juan-juan1, Su Ju-ping1, Yan Chun-lu1   

  1. 1Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China
    2Room of Integrated Chinese and Western Medicine, Gansu Province Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine, Lanzhou 73000, Gansu Province, China
  • Received:2012-01-07 Revised:2012-02-20 Online:2012-10-07 Published:2012-10-07
  • About author:Liu Yong-qi★, Master, Professor, Master’s supervisor, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China liuyongqi73@163.com

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Abstract:

BACKGROUND: There have been no reports describing the effects of cadmium chloride on heredity of bone marrow mesenchymal stem cells (BMSCs)
OBJECTIVE: To investigate the effects of cadmium chloride on micronucleus rate and chromosome aberration rate in mouse BMSCs.
METHODS: Mouse BMSCs were cultured by adherence method. Morphology of BMSCs was observed in the blank control group and cadmium chloride-induced group. The micronucleus rate and chromosome aberration rate in mouse BMSCs were determined.
RESULTS AND CONCLUSION: Under the microscope, cells in the blank control group grew adherently like fibroblasts, with transparent soma and strong refraction; in the cadmium chloride-induced group, cells were retracted, somas became small and round, and were weakly adherent, and suspension cells increased. The micronucleus rate and chromosome aberration rate in mouse BMSCs were significantly increased in the cadmium chloride-induced group than in the blank control group (P < 0.05). These findings suggest that cadmium chloride can cause damage to BMSC genetic material.

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