Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6710-6716.doi: 10.3969/j.issn.2095-4344.2012.36.011

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Effect of bone marrow mesenchymal stem cells transplantation on fibrosis in severe chronic hepatic injury induced by diethylnitrosamine

Ji Wei-zheng1, Wang Wen-ran2, Song Hao1, Wen Hao1   

  1. 1Department of Interventional Radiology, 2Tumor Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2012-05-09 Revised:2012-05-22 Online:2012-09-02 Published:2012-09-02
  • About author:Ji Wei-zheng☆, Studying for doctorate, Associate chief physician, Department of Interventional Radiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jwzgxl@sina.com

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Abstract:

BACKGROUND: Many studies have shown that bone marrow mesenchymal stem cells could improve liver fibrosis.
OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cells on fibrosis in chronic hepatic injury induced by diethylnitrosamine.
METHODS: Twenty female Wistar rats were used to establish the model with chronic hepatic injury induced by diethylnitrosamine, and then the models were randomly divided into two groups. Rats in the mesenchymal stem cells group (n=10) received 1×106/mL male bone marrow mesenchymal stem cells injection through caudal vein at 4, 8, 12 and 16 weeks, and the rats in the model group received equal volume of saline injection.
RESULTS AND CONCLUSION: At 20 weeks after chronic hepatic injury, sex-determining region of Y-chromosome was detected in mesenchymal stem cells group but not in model group. It indicated that bone marrow mesenchymal stem cells were successfully transplanted in mesenchymal stem cells group, and survived. MRI showed that a large number of regenerative nodules could be seen in mesenchymal stem cells group. Comparred with model group, the number of regenerative nodules, fibrosis score, α-smooth muscle actin and percentage of collagen area were increased in mesenchymal stem cells group (P < 0.05). Under our experimental conditions, bone marrow mesenchymal stem cells in systemic circulation leads to their engraftment into liver tissue and promotes fibrosis in rat model of severe chronic liver injury induced by diethylnitrosamine.

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