Abstract:

BACKGROUND: There are many studies describing transplantation of bone marrow mesenchymal stem cells for treatment of various inflammatory diseases. But few studies are reported on intervention of injury of organs related to severe acute pancreatitis.
OBJECTIVE: To investigate the effect of allogenetic bone marrow mesenchymal stem cells transplantation on severe acute pancreatitis associated lung injury in rats.
METHODS: Bone marrow mesenchymal stem cells were harvested by whole bone marrow adherence method. In the sham-operated group (n=32), SD rats received only light and soft tipping of pancreatic gland. In the model group (n=68), SD rats were prepared into severe acute pancreatitis models and randomly subdivided into an intervention subgroup and control subgroup. Four time points were designated for each subgroup. In the intervention subgroup, 1×10⁹/L bone marrow mesenchymal stem cells suspension was injected via the tail vein; while in the control subgroup, equal amount of physiological saline was injected. In each subgroup, one rat per time point was selected for cell tracing by CM-Dil injection.
RESULTS AND CONCLUSION: Retrograde cholangiopancereatography injection molding can induce severe acute pancreatitis and associated lung injury during the early stage. Inflammatory factor and E-selectin expression was significantly increased and pancreatic gland and lung injury was aggravated with prolonged time. After labeling, red fluorescence appeared in the lung tissue and increased with prolonged time. At each time point, lung injury was alleviated, serum amylase and tumor necrosis factor α and interleukin 1β expression was decreased in the intervention subgroup compared with the control subgroup. E-selectin expression in the intervention subgroup was significantly decreased than that in the control subgroup. Transplantation of allogenetic bone marrow mesenchymal stem cells can effectively alleviate severe acute pancreatitis associated lung injury.