Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6651-6657.doi: 10.3969/j.issn.2095-4344.2012.36.001

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Expression of vascular endothelial growth factor and bone morphogenetic protein 2 during osteogenic induction of rabbit bone marrow mesenchymal stem cells

Liu Tang, Zhang Xiang-Sheng, Zhang Qing, Lei Ting, Xiong Guang-zhong   

  1. The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
  • Received:2012-03-05 Revised:2012-04-09 Online:2012-09-02 Published:2012-09-02
  • Contact: Zhang Qing, Master, Chief physician, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China zhangqing65230@126.com
  • About author:Liu Tang☆, Doctor, Attending physician, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China liutang1204@126.com

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Abstract:

BACKGROUND: Studies have shown that vascular endothelial growth factor can promote endothelial cell proliferation, differentiation and migration, but the reports describing how the vascular endothelial cell growth factor and bone morphogenetic protein 2 express in the cells differentiated from bone marrow mesenchymal stem cells are rare.
OBJECTIVE: To observe the expression of vascular endothelial growth factor and bone morphogenetic protein 2 during osteogenic induction of rabbit bone marrow mesenchymal stem cells.
METHODS: Passage 3 New Zealand white rabbits were used for bone marrow mesenchymal stem cells extraction. The cells were divided into two groups at 1 day after extraction: the culture medium was not changed in the control group; the induction group was induced with osteogenic medium, and flow cytometry was used to detect the expression of cell surface antigen. RT-PCR and Western blot were used to detect the expression of vascular endothelial growth factor and bone morphogenetic protein 2 during osteogenic induction.
RESULTS AND CONCLUSION: During osteogenic induction of rabbit bone marrow mesenchymal stem cells, cell morphology was gradually changed from a long fusiform to short fusiform and polygonal, and finally stacked into colony growth. During the induction process, alkaline phosphatase content was gradually increased in the induction group, and there was a significant difference when compared with the control group (P < 0.05). Positive immunocytochemical staining of type Ⅰ collagen, osteocalcin and calcium node could be seen at 7, 14 and 21 days respectively. RT-PCR and Western blot showed that vascular endothelial growth factor and bone morphogenetic protein 2 were expressed in two groups, and there was significant difference at 7, 14 and 21 days after induction (P < 0.05). The expression of vascular endothelial growth factor and bone morphogenetic protein 2 peaked at 7 days after osteogenic induction of bone marrow mesenchymal stem cells. The method of isolation and culture of bone marrow mesenchymal stem cells is simple and feasible. Bone marrow mesenchymal stem cells can be induced to differentiate into osteoblasts with strong growth and proliferation abilities. During osteogenic induction of bone marrow mesenchymal stem cells, vascular endothelial growth factor and bone morphogenetic protein 2 expression first increased and then decreased. The expression of vascular endothelial growth factor may play a role in promoting angiogenesis.

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