Abstract:

BACKGROUND: Mesenchymal stem cells derived from mouse hair follicle possess multipotential differentiation ability. There have been few reports describing cell surface marker during chondrogenic induction process of mesenchymal stem cells derived from mouse hair follicle in vitro.
OBJECTIVE: To investigate cell morphology, cell surface markers and sulfate glycosaminoglycan level during chondrogenic induction of mesenchymal stem cells derived mouse hair follicle.
METHODS: IRC newborn mouse skin stem cells were in vitro isolated and cultured. Then cells were cultured with chondrogenic culture medium for 7 and 14 days for determination of indices.
RESULTS AND CONCLUSION: After treated with chondrogenic medium, the counts of CD44+ cells did not increase dramatically and the level of sulfate glycosaminoglycan was not changed, but the counts of CD54+ cells and CD166+ cells were significantly increased and the levels of corresponding sulfate glycosaminoglycans showed the same increasing tendency. These results suggest that mesenchymal stem cells derived from mouse hair follicle could differentiate into chondrocyte-like cells under the induction of medium; CD44 could not be used as the cell surface marker of chondrogenic differentiation of mesenchymal stem cells derived from mouse hair follicle, while CD54 and CD166 as well as corresponding sulfate glycosaminoglycans are competent for cell surface from marker of chondrogenic differentiation of mesenchymal stem cells derived from mouse hair follicle.