Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5072-5075.doi: 10.3969/j.issn.2095-4344.2012.27.025

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Long term intranasal delivery of bone marrow stromal cells improves rat brain injury caused by ischemia reperfusion

Wang Yang1, Shen Hui-juan2, Zhu Qun-e3, Wang Fei3, Xu Wei-zhen4, Yu Qian4, Zeng Xian-zhi5   

  1. 1School of Medicine; 2Electrical Engineering Center, Mechanical and Electrical Engineering College; 3Medical Research Center, School of Medicine; 5Department of Anatomy, School of Medicine, Jiaxing College, Jiaxing 314001, Zhejiang Province, China; 4Department of Pathology, Zhejiang Provincial People's Armed Police Corps Hospital, Jiaxing 314000, Zhejiang Province, China
  • Received:2011-12-08 Revised:2011-12-08 Online:2012-07-01 Published:2013-11-01
  • Contact: Zeng Xian-zhi, M.D., Associate professor, Department of Anatomy, School of Medicine, Jiaxing College, Jiaxing 314001, Zhejiang Province, China
  • About author:Wang Yang, School of Medicine, Jiaxing College, Jiaxing 314001, Zhejiang Province, China wydc88@sina.com

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Abstract:

BACKGROUND: Nasal delivery is a highly effective way to deliver drug into the brain, which can overcome the blockage of blood-brain barrier and peripheral side-effects.
OBJECTIVE: To explore the feasibility of long term intranasal delivery of bone marrow stromal cells for treatment of brain injury caused by ischemia reperfusion.
METHODS: Bone marrow stromal cells were sorted from rat whole bone marrow by adherent culture. Brain ischemia reperfusion rats were randomly divided into experimental group or control group. Bone marrow stromal cells or phosphate buffer saline were dropped into the nasal cavity of experimental group and control group respectively, once every 2 days for a total of 4 weeks. Rat behaviors were assessed once a week. Brain pathological examination was performed after the last behavior assessment.
RESULTS AND CONCLUSION: Compared with the control group, the results of modified neurological severity score and Morris water maze test showed that rat behaviors of experimental group were gradually improved at 2 weeks after treatment (P < 0.05, P < 0.01). It corresponded to behavior improvement, and ischemia reperfusion resulted in 66% cell loss in hippocampal CA1 region in the control group; however, in the experimental group, the cell loss in hippocampal CA1 region was significantly lower to 25% (P < 0.01). Our data indicated that long term intranasal delivery of bone marrow stromal cells can improve the brain injury caused by ischemia reperfusion.

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