Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5034-5038.doi: 10.3969/j.issn.2095-4344.2012.27.018

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Biological characteristics of epidermal stem cells and follicle stem cells

Yang Qing1, Li Xiu-lan2, Zhang Yang2, Guo Yue2, Sun Xiao-lei2   

  1. 1Tianjin Medical University, Tianjin 300070, China;
    2Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China
  • Received:2012-04-06 Revised:2012-05-02 Online:2012-07-01 Published:2013-11-01
  • Contact: Li Xiu-lan, Professor, Doctoral supervisor, Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China Lixiulan1954@sina.com

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Abstract:

BACKGROUND: More appropriate skin tissue engineering seed cells can better repair skin function.
OBEJECTIVE: To isolate and culture epidermal stem cells and follicle stem cells, and compare their biological characteristics.
METHODS: Epidermal stem cells and follicle stem cells of 2-month-old New Zealand rabbits were isolated and cultured in vitro. The biological characteristics of passage 2, 3, 6 cells which grew well were observed.
RESULTS AND CONCLUSIONS: Follicle stem cells attached faster and had higher proliferative capacity than epidermal stem cells. Immunohistochemical staining and fluorescent quantitative PCR showed that the follicle stem cells showed higher protein and mRNA expression of β1 integrin and K19 than epidermal stem cells. These findings suggest that follicle stem cells, as seed cells of skin tissue engineering, are superior to epidermal stem cells.

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