Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5017-5021.doi: 10.3969/j.issn.2095-4344.2012.27.015

Previous Articles     Next Articles

Persistent expression of pIRES-bone morphogenetic protein 2 plasmid after transfection into rat bone marrow mesenchymal stem cells

Sun Li1, Tian Xiao-bin1, Hu Ru-yin1, Wang Yuan-zheng1, Tian Jia-liang1, Han Wei1, Li Bo1, Yuan Jun2, Nie Ying-jie2   

  1. 1Department of Orthopedics, 2Central Laboratory, People’s Hospital of Guizhou Province, Guiyang 550002, Guizhou Province, China
  • Received:2012-04-19 Revised:2012-05-10 Online:2012-07-01 Published:2013-11-01
  • Contact: Tian Xiao-bin, Chief physician, Department of Orthopedics, People’s Hospital of Guizhou Province, Guiyang 550002, Guizhou Province, China txb6@vip.163.com

Abstract:

BACKGROUND: Recombinant human bone morphogenetic protein has been widely used in bone tissue engineering for treatment of bone defects or bon nonunion. But direct application of exogenous bone morphogenetic protein for repair of bone defects yields unsatisfactory effects.
OBJECTIVE: To investigate persistent secretion and expression of bone morphogenetic protein in rat bone marrow mesenchymal stem cells transfected with pIRES-bone morphogenetic protein.
METHODS: Rat bone marrow mesenchymal stem cells were isolated and cultured by the whole bone marrow adherence method. Under the liposome induction, eukaryotic expression plasmid pIRES-bone morphogenic protein 2 was introduced into rat bone marrow mesenchymal stem cells.
RESULTS AND CONCLUSION: ELISA results showed that with the prolonged transfection time of pIRES-bone morphogenetic protein, bone morphogenetic protein 2 secreted by rat bone marrow mesenchymal stem cells was gradually increased, peaked at 10-12 days after transfection, and was relatively much at 15 days after transfection. These findings suggest that after transfected by pIRES-bone morphogenetic protein 2, rat bone marrow mesenchymal stem cells can persistently and stably secrete bone morphogenetic protein 2.

CLC Number: