Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5011-5016.doi: 10.3969/j.issn.2095-4344.2012.27.014

Previous Articles     Next Articles

Hyperbaric oxygenation for theraputic angiogenesis acquired by transplantation of vascular endothelial growth factor gene modified bone marrow mesenchymal stem cells in rats with myocardial infarction

Gao Feng1, Zhang Jin-bao1, Zhou Kai1, Ouyang Hui1, Tang Ke1, Wu Xiao-chen1, He Chun-yang2, Liu Wei-yong3   

  1. 1Department of Cardiothoracic Surgery,
    2Department of Hyperbaric Oxygenation, Chengdu Military General Hospital, Chendu 610083, Sichuan Province, China;
    3Institute of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710033, Shaanxi Province, China
  • Received:2012-04-06 Revised:2012-06-04 Online:2012-07-01 Published:2013-11-01
  • Contact: Zhang Jin-bao, Professor, Department of Cardiothoracic Surgery, Chengdu Military General Hospital, Chendu 610083, Sichuan Province, China wanderer_001@163.com
  • About author:Gao Feng☆, M.D., Attending physician, Department of Cardiothoracic Surgery, Chengdu Military General Hospital, Chendu 610083, Sichuan Province, China cardiomountain@163.com

PDF

489

Abstract:

BACKGROUND: The pathological changes of bone marrow mesenchymal stem cells after transplantation into peri-infarct myocardium including ischemia, hypoxia, inflammation, and cell apoptosis greatly influence the survival of transplanted bone marrow mesenchymal stem cells, while hyperbaric oxygenation can significantly improve the hypoxic state.
OBJECTIVE: To investigate the effects of hyperbaric oxygenation for therapeutic angiogenesis acquired by transplantation of vascular endothelial growth factor gene modified bone marrow mesenchymal stemcells in rats with myocardial infarction.
METHODS: Rat bone marrow mesenchymal stem cells transfected with eukaryotic expression vector pcDNA3.1(-)/VEGF165 were transplanted into the ischemic tissue of a rat model of myocardial infarction. Prior to transplantation, rat bone marrow mesenchymal stem cells were labeled with CM-Dil. Within 2 weeks after transplantation, hyperbaric oxygenation was performed daily. At 1 month after transplantation, the left ventricular ejection fraction was measured by ultrasonic cardiogram, the density of newly formed vessels was determined by histochemical, hematoxylin-eosin, and Ⅷ factor staining.
RESULTS AND CONCLUSION: CM-DiI could label nearly 100% bone marrow mesenchymal stem cells. At 1 month after transplantation, the left ventricular ejection fraction and the density of newly formed vessels in rats subjected to hyperbaric oxygenation were significantly increased (P < 0.05). Hyperbaric oxygenation after cell transplantation results in remarkable therapeutic angiogenesis and improves cardiac function by transplantation of vascular endothelial growth factor gene-modified bone marrow mesenchymal stem cells.

CLC Number: