Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 4979-4984.doi: 10.3969/j.issn.2095-4344.2012.27.008

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Adipose derived mesenchymal stem cells differentiate into endothelial cells and construction of tissue-engineered heart valves in vitro

Zhou Hong, Zhang Tao   

  1. Department of Emergency, Beijing Coal General Hospital, Beijing 100028, China
  • Received:2011-10-21 Revised:2011-11-11 Online:2012-07-01 Published:2013-11-01
  • Contact: Zhang Tao, Master, Chief physician, Department of Emergency, Beijing Coal General Hospital, Being 100028, China

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Abstract:

BACKGROUND: Tissue-engineered heart valves are the hotspot of artificial heart valve replacement studies. It utilizes tissue-engineering technology which can construct artificial valves by planting seed cells in valve scaffolds. The current domestic and foreign studies mainly focus on the selection of seed cells and valve scaffolds.
OBJECTIVE: To explore the feasibility of constructing tissue-engineered heart valves in vitro by seeding endothelial cells differentiated from adipose derived mesenchymal stem cells in decellularized porcine aortic valve scaffolds.
METHODS: The human adipose tissue was collected by liposuction. Then the adipose derived mesenchymal stem cells were isolated and cultured, and the cell phenotype was identified by flow cytometry. The cell differentiation markers were detected by immunocytochemistry and RT-PCR. The decellularized porcine aortic valve scaffolds were made by Triton X-100 combined with trypsin digestion procedure. Tissue-engineered heart valves were constructed by seeding the endothelial cells differentiated from adipose derived mesenchymal stem cells on the decellularized porcine aortic valve scaffolds. Morphological characteristics of tissue-engineered heart valves were observed under light and electron microscopes.
RESULTS AND CONCLUTION: The endothelial cells differentiated from adipose derived mesenchymal stem cells could express endothelial markers, such as CD31, CD34, CD144 and Ⅷ factor and endothelial nitric oxide synthetase (eNOS). Porcine cells could no longer be detected on decellularized porcine aortic valve scaffolds with elastic fibers and collagenous fibers were well preserved. Endothelial cells formed a continuous monolayer on the surface of the tissue-engineered heart valves. Results indicate that the endothelial cells differentiated from adipose derived mesenchymal stem cells have some endothelial function and can proliferate well on the decellularized porcine aortic valve scaffolds. It is feasible to construct tissue-engineered heart valves in vitro by planting the endothelial cells differentiated from adipose derived mesenchymal stem cells on the decellularized porcine aortic valve scaffolds.

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