Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 4974-4978.doi: 10.3969/j.issn.2095-4344.2012.27.007

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Telomerase expression during induced differentiation of bone marrow mesenchymal stem cells into neural stem cells

Xu Tao, Yang Ming-kun, Sheng Wei-bin, Huang Kai   

  1. Department of Spine Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China
  • Received:2012-03-14 Revised:2012-06-05 Online:2012-07-01 Published:2013-11-01
  • Contact: 盛伟斌，教授，主任医师，新疆医科大学第一附属医院脊柱外科，新疆维吾尔自治区乌鲁木齐市 830054 wbsheng@vip.sina.com

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Abstract:

BACKGROUND: Telomerase plays an important role in cell differentiation and activation.
OBJECTIVE: To detect the expression of telomerase during induced differentiation of bone marrow mesenchymal stem cells into neural stem cells.
METHODS: The expression of telomerase in the former six generations of SD rat bone marrow mesenchymal stem cell subcultures was detected using the method of PCR-ELISA. Passage 3 SD rat bone marrow mesenchymal stem cells were induced to differentiate into neural stem cells and subpassaged till passage 6, and the expression of telomerase in each generation was detected. The protein extract of 293 cells of human embryo kidney cell line was used as the positive control, and protein extract from inactivated cells was used as the negative control.
RESULTS AND CONCLUSION: Telomerase showed a positive expression in the former six generations of bone mesenchymal stem cell subcultures, which was 9.4%-30.9% of the positive control groups, and the highest telomerase expression appeared in the third generation. Telomerase showed a positive expression in the former five generations of subcultures after induced-differentiation into neural stem cells, which was 5.4%-33.1% of the positive control groups. Telomerase expression peaked in the third and fourth generations of subcultures. These results suggest that telomerase is an important factor of cell differentiation and proliferation.

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