Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 4953-4958.doi: 10.3969/j.issn.2095-4344.2012.27.003

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Bone morphogenetic protein-2 induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells in vitro

Gao Chen-wei, Wang Hai-ping, Wu Zhi-gang, Lü Yang, Huo Yan-li, Wang Hao-yu   

  1. Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Received:2011-11-28 Revised:2011-12-12 Online:2012-07-01 Published:2013-11-01
  • Contact: Wang Hai-ping, M.D., Associate professor, Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, Hebei Province, China haipingmimi@126.com

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Abstract:

BACKGROUND: Many kinds of cytokines, traditional Chinese medicine monomers and tissue lysate have been used for the differentiation process by investigators. And they try to treat myocardial infarction.
OBJECTIVE: To explore the effect of bone morphogenetic protein-2 in the differentiation of rat bone marrow mesenchymal stem cells into cardiomyocyte-like cells in vitro.
METHODS: Bone marrow mesenchymal stem cells were isolated from SD rat bone marrow and cultured for 72 hours with the medium containing bone morphogenetic protein-2 (the control group was cultured with normal medium), and then cultured with normal medium for 4 weeks.
RESULTS AND CONCLUSION: After purification and culture, the cells showed CD29 positive and CD34 negative, which met the characteristics of bone marrow mesenchymal stem cells, the growth curve showed the cells had similar growtj patterns. Bone marrow mesenchymal stem cells exhibited a narrow morphology and tightly packed in the same direction after induced by bone morphogenetic protein-2. The differentiated cells were positive for C-TnI, C-TnT, desmin, α-sarcomeric actin and P38MAPK. There was no positive staining in control group. Bone morphogenetic protein-2 can induce the bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells in vitro. It is a new and effective inductor for bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells in vitro.

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