Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (5): 657-661.doi: 10.3969/j.issn.2095-4344.1912

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Difference in the effect of metformin on bone strength of lumbar vertebrae and hind limbs in diabetic rats

Yu Peiyuan1, Zhang Zhida1, Liang Lichang1, Yang Zhidong2, Huang Jinjing1, Peng Jiancheng1, Liang Ziyang1, He Jiahui1, Zhao Wenhua1,

Yu Fuyong3, Chen Guifeng3, Liang De2, Jiang Xiaobing2   

  1. 1the First School of Clinical Medicine of Guangzhou University of Chinese Medicine; 2Department of Spine, the First Affiliated Hospital of Guangzhou University of Chinese Medicine; 3the Second School of Clinical Medicine of Guangzhou University of Chinese Medicine
  • Received:2019-04-22 Revised:2019-04-30 Accepted:2019-06-14 Online:2020-02-18 Published:2020-01-08
  • Contact: Yang Zhidong, Professor, Master’s supervisor, Department of Spine, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China Jiang Xiaobing, Associate professor, Doctoral supervisor, Department of Spine, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • About author:Yu Peiyuan, Master candidate, the First School of Clinical Medicine of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81674000 and 81503591; the Second Batch of Research Project for the Construction of the National Traditional Chinese Medicine Clinical Research Base, No. 2015L01; the Natural Science Foundation of Guangdong Province, No. 2016A030313645 and 2014A030310082; the Project of Science and Technology Department of Guangdong Province, No. 2014A020221021; the Guangdong Provincial Administration of Traditional Chinese Medicine, No. 20173006; the Science and Technology Program of Guangzhou, No. 201707010298 and 201710010078

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Abstract:

BACKGROUND: Metformin has been shown to regulate bone metabolism to some extent, but its effect on bone strength is rarely reported.

OBJECTIVE: To observe the difference in the effect of metformin on the bone strength of lumbar vertebrae and hind limb in diabetic rats. 

METHODS: The study was approved by the Laboratory Animal Ethical Committee of the First Affiliated Hospital of Guangzhou University of Chinese Medicine. Six rats randomly selected from 30 rats were as blank group. The remaining 24 rats were given the intraperitoneal injection of streptozotocin (40 mg/kg) once after 6 weeks of high-sugar and high-fat diet to establish the model of type 2 diabetes mellitus. Twelve of them were successfully selected and divided into model group and metformin group. After modeling, in the metformin group, 300 mg/kg per day metformin suspension 2 mL was intragastrically administered once a day; the blank group and the model group were intragastrically administered 0.9% sodium chloride 2 mL once a day. Each group was administered for 12 weeks. The maximal load and elastic modulus of lumbar vertebrae, femur and tibia of each group were measured at 8 and 12 weeks after administration. The maximum load improvement rate of metformin on the central and peripheral bone at 12 weeks was calculated. 

RESULTS AND CONCLUSION: (1) The maximum load of the tibia was increased after 8 and 12 weeks of administration in the metformin group. The maximum load on the lumbar spine and femur was also increased after 12 weeks of administration (P < 0.05). (2) After 12 weeks of administration, the elastic modulus of lumbar vertebrae and tibia in the metformin group was higher than that in the model group (P < 0.05). (3) After 12 weeks of administration, the maximal load improvement rate of the lumbar spine in the metformin group was 38.35%, the maximum femur repair rate was 24.05%, and the maximum tibia load improvement rate was 14.70%. (4) These findings suggest that metformin has a certain improvement on the bone strength of the lumbar vertebrae and hind limb in diabetic rats, and the improvement of the bone strength on the central axis (lumbar spine) is better than that of the peripheral bone (femur, tibia).

Key words: diabetes, diabetic osteoporosis, metformin, bone strength, biomechanics

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