Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (10): 1540-1546.doi: 10.3969/j.issn.2095-4344.1886
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He Yunfei1, 2, Wang Shuang1, Ma Jun3, Yu Lei1, Wen Jiankun1, Ye Xiaojian1
Received:
2019-05-15
Revised:
2019-06-03
Accepted:
2019-06-27
Online:
2020-04-08
Published:
2020-02-15
Contact:
Ye Xiaojian, Chief physician, Department of Spinal Minimally Invasive Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
About author:
He Yunfei, MD, Attending physician, Department of Spinal Minimally Invasive Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Department of Orthopedics, the 940 Hospital of People's Liberation Army Strategic Support Force, Lanzhou 730000, Gansu Province, China
Wang Shuang, Studying for doctorate, Attending physician, Department of Spinal Minimally Invasive Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
Both He Yunfei and Wang Shuang contributed equally to this paper.
Supported by:
CLC Number:
He Yunfei, Wang Shuang, Ma Jun, Yu Lei, Wen Jiankun, Ye Xiaojian. P38/Akt pathway regulates the oriented differentiation of bone marrow mesenchymal stem cells in nanofiber annulus fibrosus scaffolds with different spatial structures[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(10): 1540-1546.
2.2 细胞黏附、增殖行为的检测 骨髓间充质干细胞在3种支架中培养后,在2 h时三者细胞黏附情况无显著差异,随时间延长,细胞的黏附效率均增加,4,8 h后AFS、AYS组细胞黏附效率仍无显著差异,而3-DPS组黏附率更高,可以认为3种材料中骨髓间充质干细胞均可良好黏附,但随时间延长,3-DPS的黏附效率高于其余两组,见图3A。继续延长培养时间,在1,3,7 d时以CCK-8法评估细胞在3种支架中的增殖情况,可以看出骨髓间充质干细胞在3种支架中均可良好增殖,在1 d时3-DPS组增殖情况差于另外两组,而在7 d时则明显优于其他两组,而AFS、AYS组在1,3,7 d时增殖情况均无明显差异,见图3B。 "
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