Co-culture model of bone marrow mesenchymal stem cell sheet and umbilical vein endothelial cells: establishment and identification

doi:10.3969/j.issn.2095-4344.1773

Abstract

Abstract:

BACKGROUND: Cell sheet technology is a recent popular technique for tissue engineering. However, a failure is prone to occur because of poor nutrient supply in the middle part of the membrane and post-transplantation blood supply, so pre-vascularization determines the survival rate of tissue engineering materials after transplantation.

OBJECTIVE: To investigate the pre-vascularization of bone marrow mesenchymal stem cell sheets co-cultured with human umbilical vein endothelial cells under different oxygen tensions.

METHODS: Bone marrow mesenchymal stem cell sheets were prepared under physiological hypoxia (2% O₂) and normoxia (20% O₂), and then co-cultured with human umbilical vein endothelial cells under normoxia. At 7 days of co-culture the angiogenesis was observed and compared by immunofluorescence staining. The level of vascular endothelial growth factor was compared under different oxygen tensions using ELISA.

RESULTS AND CONCLUSION: Compared with the normoxia co-culture group, there were longer microvessels, higher vessel density and more microvessel networks in the hypoxia co-culture group (P < 0.05). The expression of vascular endothelial growth factor in the hypoxia co-culture group was higher than that in the normoxia co-culture group (P < 0.05). Therefore, the hypoxia co-culture is more beneficial to the growth of microvessels than the normoxia co-culture, and it is feasible to construct an optimized prevascularized cell sheets.

Key words: hypoxia, normoxia, cell sheet, bone marrow mesenchymal stem cells, immunofluorescence staining, umbilical vein endothelial cells, vascular endothelial growth factor, stem cells, vascularization, tissue engineering

CLC Number:

R453
R364

Zhou Zhen, Wang Yamin, Ren Fei, Zhang Zhaoqiang, Zeng Shuguang, Yang Xi.

Co-culture model of bone marrow mesenchymal stem cell sheet and umbilical vein endothelial cells: establishment and identification

[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(25): 3951-3955.

Figures/Tables 2

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