Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (18): 2900-2908.doi: 10.3969/j.issn.2095-4344.1728
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Received:
2019-02-19
Online:
2019-06-28
Published:
2019-06-28
Contact:
Fu Songtao, Professor, School of Basic Sciences, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Shanxi Binda Stem Cell Technology Co., Ltd., Taiyuan 030001, Shanxi Province, China; Shanxi Provincial Biomedical Health Graduate Education Innovation Center, Taiyuan 030001, Shanxi Province, China
About author:
Li Jingjing, Master candidate, School of Basic Sciences, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
CLC Number:
Li Jingjing, Guo Xuan, Xie Jun, Huang Lei, Suo Jinrong, Fu Songtao. Preparation of agarose/gelatin/hyaluronic acid/extracellular matrix hydrogel and its property characterization[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(18): 2900-2908.
2.2 水凝胶支架及细胞外基质颗粒的超微结构 扫描电镜观察表明:4组样品均呈现高度互连且松散的不规则多孔网络结构,未添加细胞外基质颗粒水凝胶的凝胶孔径较均匀,并且孔隙光滑;添加细胞外基质颗粒后,通过光的透射情况可观察到水凝胶的凝胶孔径显著增大,并且孔壁逐渐变得粗糙,见图2,这可能是由于添加的细胞外基质颗粒本身是疏松多孔的结构,加入到水凝胶溶液后,部分水凝胶向细胞外基质颗粒内部多孔结构迁移,导致游离的水凝胶含量减少,即可形成水凝胶支架孔壁的含量减少,因而,水凝胶的凝胶孔径显著增大。孔壁变粗糙可能是较多的细胞外基质颗粒黏附在变薄的孔壁上造成的。以上结果说明细胞外基质颗粒的添加,会改变琼脂糖/明胶/透明质酸水凝胶的孔径大小和孔隙粗糙程度,改变其三维网络结构,故为确保水凝胶结构的稳定性,加入细胞外基质颗粒的量不能太高。"
2.5 水凝胶的生物力学强度测试 加入适量细胞外基质颗粒,琼脂糖/明胶/透明质酸水凝胶的应力-应变行为明显得到了改善。当添加细胞外基质颗粒质量浓度为0-20 g/L时,随着细胞外基质颗粒质量浓度的增加,琼脂糖/明胶/透明质酸水凝胶的生物力学强度逐渐增大;当添加的细胞外基质颗粒质量浓度为20 g/L时,琼脂糖/明胶/透明质酸水凝胶拥有较大的耐压力(约为0.06 MPa)和较强的抵制变形能力;当添加细胞外基质颗粒的质量浓度继续增加到30 g/L时,琼脂糖/明胶/透明质酸水凝胶的最大耐压力和抵制变形的能力反而表现出减弱趋势,见图6。这可能是由于细胞外基质颗粒的添加,改变了琼脂糖/明胶/透明质酸水凝胶的交联度,造成其脆碎度增加,易产生碎裂现象。以上研究表明通过调控细胞外基质颗粒的添加量,可有效改善琼脂糖/明胶/透明质酸水凝胶的生物力学性能。"
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