Resveratrol combined with endothelial progenitor cell transplantation for treatment of cadmium-induced renal injury in rats

doi:10.3969/j.issn.2095-4344.1689

Abstract

Abstract:

BACKGROUND: Studies have shown that resveratrol can effectively improve the survival rate of rat proximal tubular epithelial cells and inhibit cell apoptosis to protect against renal tubular epithelial cell injury. However, the specific mechanism of resveratrol in the treatment of renal injury in rats remains to be further clarified.
OBJECTIVE: To investigate the protective effect and mechanism of resveratrol combined with endothelial progenitor cell transplantation on renal injury caused by chronic cadmium exposure in rats.
METHODS: Rat endothelial progenitor cells provided by the Chinese Academy of Medical Sciences were resuscitated and cultured in vitro, and cell suspensions were then prepared. Endothelial progenitor cells were labeled with CM-Dil. Wistar rats purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. in China were randomly divided into control group, pure cadmium poisoning group (model group), resveratrol group, endothelial progenitor cell group and combined treatment group, with 15 rats in each group. In the control group, physiological saline was injected subcutaneously. In the other four groups, the rats were subcutaneously administered with 6 μmol/kg cadmium chloride once daily for 6 weeks to establish animal models of renal injury. After modeling, the rat models in the latter four groups were given 0.5 mL of L-DMEM via the tail vein, 0.5 mL of endothelial progenitor cell suspension (1×10¹⁰ cell/L) via the tail vein, 120 mg/kg resveratrol via gavage or endothelial progenitor cell suspension plus resveratrol. The administration in each group was taken once a day for 7 consecutive days.
RESULTS AND CONCLUSION: (1) Compared with the model group, the levels of urea nitrogen, creatinine, urinary acetylglucosaminidase, urinary γ-L-glutamyl transpeptidase and urine protein decreased significantly in the resveratrol group, endothelial progenitor cell group (P < 0.05) and dramatically in the combined treatment group (P < 0.01). (2) Compared with the model group, the levels of reactive oxygen species and malondialdehyde were reduced in the resveratrol group and endothelial progenitor cell group, whereas the activities of superoxide dismutase and glutathione peroxidase increased significantly (P < 0.05). Compared with the other groups, the combined treatment group had a greater improvement in the above-mentioned renal tissue indicators (P < 0.01). (3) The histopathological improvement of the renal tissue in the combined treatment group was markedly better than that in the endothelial progenitor cell group, resveratrol and model group. Under the fluorescence microscopy, the number of CM-Dil-labeled endothelial progenitor cells was significantly higher in the combined treatment group than the endothelial progenitor cell group (P < 0.05). To conclude, resveratrol combined with endothelial progenitor cell transplantation protects against renal injury induced by chronic cadmium exposure.

Key words: Cell Transplantation, Cadmium, Kidney, Tissue Engineering

CLC Number:

R453
R364

He Lijuan, Ma Chunlei, Shen Yulan, Zhao Guohong. Resveratrol combined with endothelial progenitor cell transplantation for treatment of cadmium-induced renal injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(13): 2055-2060.

Figures/Tables 4

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