Effect of clobetasol propionate on the differentiation of nasal mucosa-derived ectomesenchymal stem cells into oligodendrocyte-like cells and its mechanism

doi:10.3969/j.issn.2095-4344.1542

Abstract

Abstract:

BACKGROUND: Clobetasol propionate is a commonly used glucocorticoid in clinics. Nasal mucosa-derived ectomesenchymal stem cells (EMSCs) have the potential to differentiate into oligodendrocyte-like cells in vitro. However, the underlying mechanisms by which clobetasol propionate promote the differentiation into oligodendrocyte-like cells are unknown as yet.
OBJECTIVE: To observe the effect of clobetasol propionate on the differentiation of nasal mucosa-derived EMSCs into oligodendrocyte-like cells and to explore its possible mechanisms.
METHODS: Nasal mucosa-derivedEMSCs were isolated and cultured by adherent screening method, and cell properties and purity were identified using immunofluorescence staining. Cell proliferation of EMSCs was detected by MTT after intervention with 0-10 μmol/L clobetasol propionate. Passage 3 nasal mucosa-derived EMSCs grew well and were induced to differentiate into oligodendrocyte-like cells in the serum-free medium containing clobetasol propionate (0, 2.5 and 5 μmol/L). Cell morphology was then observed by inverted microscope. Expression of oligodendrocyte-associated proteins, myelin basic protein and oligodendrocyte transcription factor 2, was measured by immunofluorescence and western blot assay. Expression of SHH related to the SHH signaling pathway was detected by western blot assay.
RESULTS AND CONCLUSION: EMSCs cultured in vitro highly expressed vimentin and nestin. Clobetasol propionate at a low concentration promoted the proliferation of EMSCs; and on the contrary, clobetasol propionate at a high concentration inhibited the cell proliferation. Therein, 5 μmol/L was the best concentration of clobetasol propionate for cell proliferation. After 3 days of induction, a multi-polarization pattern appeared in the clobetasol propionate group, and the expressions of MBP, OLIG2 and SHH were significantly higher than those in the control group. To conclude, clobetasol propionate may promote the differentiation of EMSCs into oligodendrocyte-like cells via the SHH signaling pathway.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Clobetasol, Nasal Mucosa, Mesenchymal Stem Cells, Oligodendroglia, Cell Differentiation, Tissue Engineering

CLC Number:

Wang Qiuping, Xu Zhonghua, Bian Lu, Zhao Jun, Chen Changsheng, Wang Zhen, Shi Wentao, Zhang Zhijian. Effect of clobetasol propionate on the differentiation of nasal mucosa-derived ectomesenchymal stem cells into oligodendrocyte-like cells and its mechanism[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(5): 779-783.

Figures/Tables 1

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