Comparison of the effect and stability of rigid or flexible external fixator in inducing healing tissue formation at fracture site in mice

doi:10.3969/j.issn.2095-4344.1452

Abstract

Abstract:

BACKGROUND: Internal fixation with intramedullary nails is the most commonly used technique for closed femoral fractures, but external fixators are needed to stabilize open fractures in order to maintain rotational stability. Because the load acting on the fracture and the stability of the fixator determine the relative displacement of the broken end of the bone, the device may affect the physiological load of the operative limb, and then affect the healing.

OBJECTIVE: To compare the effects of different stiffness external fixators on the formation and stability of fracture healing tissues in mice, in order to find a new way of effective fracture fixation treatment.

METHODS: We introduced such a model using rigid and flexible external fixators with considerably different stiffness (axial stiffness of 17.9 and 0.76 N/mm, respectively). Both fixators were used to stabilize a 0.5 mm osteotomy gap in the femur of C57B/6 mice. Three-point bending tests, μCT, and histomorphometry demonstrated a different healing pattern after 21 days.

RESULTS AND CONCLUSION: (1) Flexural stiffness of the rigid group was significantly reduced by 28.1% compared with the control intact bone, while flexural fixation reduced the flexural rigidity to 18.9% of the intact bone with no significant difference between the two groups after 21 days (P > 0.05). (2) Compared with the rigid group, the volume of the whole callus of the flexible group was more than doubled, and the maximum diameter also significantly increased (P < 0.05); while the bone volume fraction significantly decreased (P < 0.05). (3) Histological analysis showed that the numbers of braided bone, laminar bone and cartilage in the callus in the flexible group were significantly higher than those of the rigid group (P < 0.05). (4) The selected rigid and flexible fixations with different stiffness both lead to callus formation, but healing is delayed under flexible fixation.

Key words: fractures, mice, external fixators, stiffness, flexibility, fracture healing, stability, braided bone, layered bone, cartilage

CLC Number:

R459.9|R318|R683.4

Li Hongyuan, Wang Liang. Comparison of the effect and stability of rigid or flexible external fixator in inducing healing tissue formation at fracture site in mice[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(28): 4510-4515.

Figures/Tables 2

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