Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (26): 4154-4159.doi: 10.3969/j.issn.2095-4344.1354

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Effect of different techniques on the osteogenic property of undecalcified heterogeneous dentin particles

Zhao Binbin1, Qiu Zewen2, Ma Chenghui3, Zhong Weijian1, Ma Guowu1
  

  1. 1Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian 116023, Liaoning Province, China; 2Laboratory Animal Center of Dalian Medical University, Dalian 116044, Liaoning Province, China; 3Department of Dentistry, the First Hospital of Jiaxing, Jiaxing 314000, Zhejiang Province, China
  • Received:2019-03-26
  • Contact: Zhong Weijian, Associate professor, Master’s supervisor, Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian 116023, Liaoning Province, China
  • About author:Zhao Binbin, Master candidate, Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian 116023, Liaoning Province, China
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 2015020313 (to ZWJ)

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Abstract:

BACKGROUND: Autologous dentin particles have been shown to possess good biocompatibility and osteoconductivity, which can be used as bone grafting materials, but the biosafety and osteogenic capacity of heterogeneous dentin particles are still unclear.
OBJECTIVE: To observe tissue reaction and osteogenic effects of undecalcified heterogeneous human dentin particles processed with different treatment methods and implanted into skull defect of rat model.
METHODS: Extracted human teeth were prepared into 0.25-0.5 mm sized dentin particles after washing, pulverizing, boiling, degreasing and sterilization (donors consent to the experiment). Forty healthy adult male Sprague-Dawley rats were selected to create an animal model of skull defect (provided by Laboratory Animal Center of Dalian Medical University), and the cortical skull bone was removed by round bur perforation. The animals were randomly divided into four groups: untreated, boiled treatment, degreased treatment, and boiled plus degreased groups. Specimens were harvested at 4 and 12 weeks after bone grafting, and the degree of inflammatory reaction and bone regeneration effects were evaluated by gross observation, histological examination and histomophometric analysis. This study was approved by the Experimental Animal Ethics Committee of Dalian Medical University, China.
RESULTS AND CONCLUSIONS: (1) Gross observation showed that the dentin particles in the four groups were surrounded by fibrous tissue, and the graft was firm and stable. At 12 weeks, the graft was slightly smaller than that at 4 weeks. (2) Histological observation showed that in each group, the fibrous connective tissue was surrounded by dentin particles and inflammatory cell infiltration and a large number of new blood vessels were observed in fibrous connective tissue. Polynuclear macrophages were visible around the dentin particles, and there were worm-like absorption regions at the edge of the particles. Compared with the 4th week, the number of new capillaries around the dentin particles increased at 12 weeks, the number of inflammatory cells decreased, new bone formation was observed around the dentin particles, and it was connected with the skull, and the osteocyte proliferated actively. Histological analysis showed that there were no significant differences in the number of inflammatory cells, neovascularization, macrophage count and new bone formation between the four groups (P > 0.05). (3) These results showed that human dentin granule treated by different methods showed similar inflammatory responses in bone grafting, and good biocompatibility and osteoconductivity in rat skull defect repair.

Key words: dentin particles, boiling method, isopropanol, bone regeneration, biological materials, animal experiments, bone graft materials, osteogenesis

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