Application of electrospun polycaprolactone-polyethylene glycol-polycaprolactone fiber scaffolds in bone tissue engineering

doi:10.3969/j.issn.2095-4344.1269

Abstract

Abstract:

BACKGROUND: Polycaprolactone-polyethylene glycol-polycaprolactone (PCL-PEG-PCL) is a good scaffold material for tissue engineering. Little is reported on electrospun PCL-PEG-PCL in bone tissue engineering.

OBJECTIVE: To prepare PCL-PEG-PCL scaffolds using electrospinning technology and investigate their physical/chemical/biological/ osteogenic properties.

METHODS: PCL-PEG-PCL scaffolds were prepared using electrospinning technology. Scaffold surface was characterized by scanning electron microscopy. The physical and chemical properties, such as the hydrophilic angle and Young’s modulus, of the scaffold were determined. Bone marrow mesenchymal stem cells were seeded on PCL-PEG-PCL scaffolds and cell morphology was observed. In the experimental group, bone marrow mesenchymal stem cells were seeded on PCL-PEG-PCL scaffolds and then cultured. In the control group, bone marrow mesenchymal stem cells were concurrently cultured without seeding on the PCL-PEG-PCL scaffolds. After culture for 1, 3, 5 days, cell proliferation was determined by the Cell Counting Kit-8 assay. After osteogenic induction for 1, 5, 7, and 14 days, the expression levels of osteogenic genes Runx2 and osteopontin were detected by fluorescence-based quantitative PCR. The study was approved by the Ethics Committee of West China School of Stomatology, Sichuan University (SKLODLL2013A173).

RESULTS AND CONCLUSION: Electrospun PCL-PEG-PCL scaffold had a non-woven structure. Its fibers were continuous and had smooth surface without granular nodules. There was no obvious boundary between the fibers. The fibers were interwoven with each other to form a three-dimensional structure with different sized pores. The hydrophilic angle was (116.1±2.5)° and was relatively hydrophobic. The Young's modulus was 16.464 4 MPa. A large number of bone marrow mesenchymal stem cells adhered on the electrospun PCL- PEG-PCL scaffolds. Adjacent cells began to connect and fuse with each other, and some of them grew into the pore of the scaffold fibers. The proliferation speed of cells cultured at different time points in the experimental group was faster than that in the control group (P < 0.05). At 5, 7, 14 days of culture, Runx2 gene expression in the experimental group was significantly higher than that in the control group (P < 0.05). At 1, 5, 7, 14 days of culture, osteopontin expression in the experimental group was significantly higher than that in the control group (P < 0.05). These results suggest that electrospun PCL-PEG-PCL scaffolds exhibit encouraging physical/chemical/biological/osteogenic properties.

Key words: polycaprolactone-polyethylene glycol-polycaprolactone, PCL-PEG-PCL, electrospinning, bone marrow mesenchymal stem cells, biocompatibility, biosafety, osteogenic gene, bone tissue engineering

CLC Number:

Fu Na, Luo Xiaoding, Jiao Tiejun, Sui Lei. Application of electrospun polycaprolactone-polyethylene glycol-polycaprolactone fiber scaffolds in bone tissue engineering[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(22): 3445-3450.

Figures/Tables 4

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