Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (26): 4101-4109.doi: 10.3969/j.issn.2095-4344.0932

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Magnesium phosphate/collagen peptide composite cements: the setting retardation effect of collagen peptides

Cao Xiao-feng1, 2, Wang Yi-hu1, 2, 3, Lu Hao-jun1, 2, Ma Ming1, 2, Guo Yan-chuan1, 2, 3, Mao Ke-ya4, Li Jiang-tao5   

  1. 1Key Laboratory of Photochemical Conversion and Optoelectronic Material, 5Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China; 2Biomaterials Research Center, Hangzhou Branch of Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Hangzhou 310018, Zhejiang Province, China; 3University of Chinese Academy of Sciences, Beijing 100049, China; 4Department of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2018-01-16
  • Contact: Guo Yan-chuan, Ph.D., Professor, Doctoral supervisor, Key Laboratory of Photochemical Conversion and Optoelectronic Material, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • About author:Cao Xiao-feng, Ph.D., Assistant professor
  • Supported by:

    the National Natural Science Foundation of China, No. 51372276, 21506236; Science and Technology Service Network Initiative of the Chinese Academy of Sciences, No. KFJ-STS-ZDTP-016

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Abstract:

BACKGROUND: Magnesium phosphate cement (MPC) is a kind of bone repair material with excellent biocompatibility and bioactivity, which has overcome some disadvantages of calcium phosphate cements. However, the MPC still has some drawbacks such as the fast reaction rate in the setting process, the short initial and final setting times, and the high temperature in the setting process. Collagen peptides (CP) as polypeptide mixtures have the properties of enhancing bone mineral density, and preventing and treating osteoarthritis and osteoporosis. So far, there are few reports on the composite cements composed of MPC and CP. The physicochemical properties and cytotoxicity of the composites cements have not been well documented.

OBJECTIVE: To prepare the MPC/CP composite cement with controllable setting rate and setting reaction heat and to study the physicochemical properties and cytotoxicity of the composite cement.
METHODS: The MPC were prepared by combining low-activity MgO, KH2PO4, and Na2B4O7•10H2O. Different amounts of CP were introduced into the MPC to form MPC/CP composite cements. We measured the initial and final setting times, the temperature evolution during the setting process and the compressive strength of the composite cements soaked in the simulated body fluid for 1 and 7 days. The MTT assay was used to evaluate the cytotoxicity of the composite cements.

RESULTS AND CONCLUSION: The addition of CP altered the physicochemical and biological properties of the composite bone cements. With the increasing of the CP content, prolonged initial and final setting times, reduced maximum temperatures during the setting process, and delayed time for the maximum temperature were observed. The hydrated and cured products of MPC and the composite bone cement were MgKPO4•6H2O. The above experimental results suggested that the CP had a good retarding effect on the composite bone cement without changing the phase of the cured product. Experimental findings regarding the mechanical properties showed that with the increasing of the CP content, the compressive strengths of the composite cements increased firstly and then decreased. The storing time in the simulated body fluid was also the influencing factor for the compressive strength of the composite cement. Results from the MTT assay showed that the addition of CP promoted the proliferation of MC3T3-E1 cells and L929 cells. Overall findings provide a technical basis for the clinical application of MPC/CP composite cements.

 

Key words: Magnesium Compounds, Biocompatible Materials, Tissue Engineering

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