Suturing and bonding for repair of annulus fibrosus defects in goats

doi:10.3969/j.issn.2095-4344.0859

Abstract

Abstract:

BACKGROUND: At present, there is a lack of effective strategies that deal with annulus fibrosus defects in a majority of cases undergoing lumbar discectomy, which is one of the important causes of postoperative relapse of lumbar disc herniation.

OBJECTIVE: To explore the effects of direct suturing and medical fibrin bonder for annulus fibrosus defects in goats undergoing resection of intervertebral discs.

METHODS: L_1/2-L_6/7 samples (36 lumbar vertebrae) from six goats aged 6 months were randomized into three groups (n=12 per group): direct suture group, bonding group and control group (with no intervention). An annular incision of 4 mm in length was made on the disc using a circle cutter. A small amount of nucleus pulposus (about 0.1 g) was taken from each sample followed by direct suturing, bonding and no treatment in corresponding groups. X-ray and MRI observation of the lumbar vertebra was performed preoperatively and 12 weeks postoperatively. Height index of the lumbar intervertebral disc (DHI) was calculated to evaluate the lumbar degeneration. The goats were sacrificed at 12 weeks postoperatively and the defect sites were observed using light microscope and transmission electron microscope.

RESULTS AND CONCLUSION: The DHI values in three groups at 12 weeks postoperatively were ranked as follows: direct suturing group > bonding group > control group. Lumbar T2WI MRI modified Pfirrmann grade was ranked as follows: direct suturing group < bonding group < control group. Histological Masuda degeneration grade was ranked as follows: direct suturing group < bonding group < control group. Performance of cell ultrastructure at repair site was ranked as follows: direct suturing group > bonding group > control group. To conclude, both direct suturing and fibrin bonder can effectively repair annulus fibrosus defects, and moreover, direct suturing is superior to bonding in the repair effect.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Intervertebral Disk, Suture Techniques, Bone Cements, Goats, Tissue Engineering

CLC Number:

R318

Wang Yu-peng, Yin He-ping, Wu Yi-min, Li Shu-wen, Du Zhi-cai, Bai Ming, Meng Ge-dong. Suturing and bonding for repair of annulus fibrosus defects in goats[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(26): 4156-4161.

Figures/Tables 6

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