Polymorphisms of interleukin-23 receptor gene and susceptibility of bone and joint tuberculosis in Guangxi Zhuang populations

doi:10.3969/j.issn.2095-4344.0840

Abstract

Abstract:

BACKGROUND: The incidence of tuberculosis is high in populations from Guangxi Zhuang Autonomous Region. Tuberculosis has been shown to have susceptibility gene.
OBJECTIVE: To analyze the relationship between interleukin-23 receptor (IL-23R) gene polymorphism and susceptibility to bone and joint tuberculosis in Guangxi Zhuang populations.
METHODS: Totally 125 patients with bone and joint tuberculosis admitted in the Affiliated Hospital of Youjiang Medical University for Nationalities and 150 healthy subjects were selected. The gene polymorphism of IL-23R (rs10489628G/A, rs10489629T/C, rs10889675A/C, rs10889677C/T, rs7530511C/A) was analyzed by PCR amplification technique and DNA sequencing method. The genotypes and frequency distribution of alleles were compared between the tuberculosis and control groups. The relationship between IL-23R gene polymorphism and the susceptibility to bone and joint tuberculersis was discussed.
RESULTS AND CONCLUSION: The frequency of C allele of IL-23R rs10489629 T/C in the tuberculosis group was 29.2%, which was significantly higher than that in the control group (21.3%) (P < 0.05). The frequency of A allele of IL-23R rs10889675 C/A in the tuberculosis group was 26.0%, which was significantly higher than that in the control group (18.0%). The frequency of CA genotype of IL-23R rs10889675 C/A in the tuberculosis group was 37.6%, which was significantly higher than that in the control group (25.3%) (P < 0.05). The frequency of GTCAC haplotype in the bone and joint tuberculosis group was 69.6% which was lower than that in the control group (77.3%). The frequency of ACACC haplotype in the bone and joint tuberculosis group was 26.0% which was higher than that in control group (17.7%). In summary, the gene polymorphism of IL-23R rs10489629T/C and rs10889675C/A may be related to the susceptibility of bone and joint tuberculosis in Guangxi Zhuang populations.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Interleukin-23, Genetic Predisposition to Disease, Tuberculosis, Osteoarticular, Tissue Engineering

CLC Number:

R318

Wu Zhao-yuan1, Xie Ke-gong2, Lu Lu2, Lu Xian-zhe2, Huang Ke2, Liao Lin-bo1, Tang Yu-jin2 . Polymorphisms of interleukin-23 receptor gene and susceptibility of bone and joint tuberculosis in Guangxi Zhuang populations[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4434-4439.

Figures/Tables 1

2.1 受试者数量分析病例组和对照组所有参与者均进入结果分析。2组研究对象性别、年龄差异均无显著性意义(P > 0.05；表2)。 2.2 白细胞介素23受体基因各位点的检测结果 ABI3730XL测序仪的SNP结果显示，rs10489628位点的基因型有3种，分别是GG、GA、AA；rs10489629位点的基因型有3种，分别是TT、TC、CC；rs10889675位点的基因型有3种，分别是AA、AC、CC；rs10889677位点的基因型有3种，分别是CC、CT、TT；rs7530511位点的基因型有3种，分别是CC、CA、AA。 2.3 Hardy-Weinberg遗传平衡检测经χ2检验，2组研究对象在该群体均存在Hardy-Weinberg平衡(P > 0.05)。 2.4 白细胞介素23受体基因单核苷酸多态性与骨关节结核易感性的关系如表3所示，骨与关节结核组白细胞介素23受体基因 rs10489629 T/C位点TT、TC、CC基因型频率分别为51.2%，39.2%，9.6%，对照组TT、TC、CC基因型频率分别为62.7%，32.0%，5.3%，两组基因型分布差异无显著性意义(P > 0.05)。骨与关节结核组白细胞介素23受体基因 rs10489629 T/C位点T、C等位基因频率分别为70.8%、29.2%，对照组T、C等位基因频率分别为78.6%、21.3%，两组等位基因频率分布差异有显著性意义(P < 0.05)，即骨与关节结核组C等位基因频率显著高于对照组，提示C等位基因(OR=0.657 5，95%CI：0.446-0.969 3)是骨与关节结核病发病易感因素，推测该位点多态性与广西壮族人群骨关节结核的易感性显著相关。骨与关节结核组白细胞介素23受体基因rs10889675 C/A位点CC、CA、AA基因型频率分别为55.2%，37.6%，7.2%，对照组CC、CA、AA基因型频率分别为69.3%，25.3%，5.3%，两组基因型分布差异有显著性意义(P < 0.05)，其中CA基因型是骨与关节结核的易感因素，该位点多态性与广西壮族人群骨与关节结核的易感性显著相关。骨关节结核组C、A等位基因频率分别为74.0%、26.0%，对照组C、A等位基因频率分别为82%、18%。骨与关节结核组A等位基因频率显著高于对照组，提示A等位基因(OR=0.624 8，95%CI：0.415 4- 0.939 7)是骨与关节结核病发病易感因素，推测该位点多态性与广西壮族人群骨与关节结核的易感性显著相关。两组余下白细胞介素23受体基因rs10489628 G/A、rs10889677A/C、rs7530511C/T位点基因型及等位基因频率分布差异无显著性意义(P > 0.05)，推测该位点多态性与广西壮族人群骨关节结核的易感性无显著相关。 2.5 白细胞介素23受体基因单倍型分析及与骨关节结核易感性的关系应用Haploview 4.2软件对骨关节结核组"

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