Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (34): 5484-5489.doi: 10.3969/j.issn.2095-4344.0684

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Leukocyte- and platelet-rich fibrin promotes wound healing

Wu Yuxiang1, Si Shaoyan2, Xu Zhangrong3, Wang Aihong1, 3   

  1. 1Department of Endocrinology, the 306th Hospital of PLA, Clinical Medicine College of Anhui Medical University, Beijing 100101, China; 2Special Medical Laboratory and Research Center, Beijing 100101, China; 3Department of Endocrinology, the 306th Hospital of PLA, Beijing 100101, China
  • Received:2018-06-29 Online:2018-12-08 Published:2018-12-08
  • Contact: Wang Aihong, MD, Master’s supervisor, Department of Endocrinology, the 306th Hospital of PLA, Clinical Medicine College of Anhui Medical University, Beijing 100101, China; Department of Endocrinology, the 306th Hospital of PLA, Beijing 100101, China
  • About author:Wu Yuxiang, Master candidate, Department of Endocrinology, the 306th Hospital of PLA, Clinical Medicine College of Anhui Medical University, Beijing 100101, China
  • Supported by:

    the PLA Medical Science and Technology Project for Youth Cultivation, No. 16QNP041; a grant from Beijing Municipal Science & Technology Commission, No. Z141107002514181; the Clinical Medical Research Special Project of Chinese Medical Association, No. 12020580358

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Abstract:

BACKGROUND: Leukocyte- and platelet-rich plasma (L-PRP) and leukocyte- and platelet-rich fibrin (L-PRF) are two kinds of autologous platelet rich gels that can accelerate wound healing.

OBJECTIVE: To compare the differences in the composition, structure and release of growth factors between L-PRP and L-PRF.
METHODS: Fasting blood samples were collected from healthy volunteers to prepare L-PRP and L-PRF gels. The microstructure of the gels was observed by light microscope and scanning electron microscope, and the concentration times and recovery rates of leukocytes and platelets were calculated. The two gels were stored in DMEM medium. After 1, 3, 7, 14 and 21 days, the culture fluid containing exudate was collected. The platelet-derived growth factor, transforming growth factor β1, and vascular endothelial growth factor were detected by ELISA.
RESULTS AND CONCLUSION: (1) Under the light microscope, platelet, leukocytes and erythrocytes were clearly demarcated, and leukocytes and fibrin aggregated in cluster. The platelets and leukocytes in the L-PRP gel were less in number and dispersed among erythrocytes. (2) Under the electron microscope, a large amount of fibrins were in the L-PRF gel, to form a dense special 3D structure, and the platelet superposition structure at the junction was clear. There were less fibers and a large number of platelets at the junction of L-PRP gel, and some of the platelets were cleaved. (3) Compared with the L-PRP gel, the L-PRF had a higher platelet recovery rate (P=0), leukocyte recovery rate (P=0) and lymphocyte recovery rate (P=0). (4) In addition to the vascular endothelial cell growth factor, the levels of transforming growth factor β1 and platelet-derived growth factor in the L-PRF gel stored for 3, 7, 14 and 21 days were higher than those in the L-PRP gel. These findings indicate that compared with the L-PRP gel, the L-PRF gel is easier to prepare, has better structure and slower release of growth factors, which have more advantages in the treatment of wounds. 

Key words: Platelet-Rich Plasma, Fibrin, Wound Healing, Tissue Engineering

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