Compatibility of nasal mucosa-derived ectomesenchymal stem cells and calcium phosphate cement in vitro

doi:10.3969/j.issn.2095-4344.0670

Abstract

Abstract:

BACKGROUND: The use of calcium phosphate cement (CPC) carrying mesenchymal stem cells for the treatment of bone defects has achieved satisfactory results. The in vitro compatibility of ectomesenchymal stem cells (EMSCs) derived from the nasal mucosa with CPC is unknown.
OBJECTIVE: To investigate the effect of calcium phosphate cement on the differentiation of rat nasal mucosa-derived EMSCs into osteoblasts and to further explore the biocompatibility of EMSCs and CPC.
METHODS: EMSCs were cultured, expanded in vitro and implanted onto the CPC surface or onto culture plates. Then, implanted EMSCs were induced to differentiate into osteoblasts for 14 days. Alkaline phosphatase activity assay was used to detect the alkaline phosphatase activity of EMSCs. The area of calcium nodules was determined by alizarin red staining. The expression level of osteogenesis-related proteins was detected by western blot assay and immunofluorescence method. Morphology of EMSCs on the CPC surface was dynamically observed using phalloidin staining. MTT assay was used to detect the proliferation of EMSCs on the CPC surface.
RESULTS AND CONCLUSION: After 14 days of osteogenic induction, there was higher alkaline phosphatase activity, larger calcium nodules and higher levels of osteogenesis-related proteins in the EMSCs implanted on the surface of CPC than those implanted on the culture plates. The CPC had a certain role in promoting the proliferation of EMSCs. To conclude, EMSCs and CPC scaffolds have good biocompatibility, and moreover, CPC can certainly promote the differentiation of EMSCs into osteoblasts.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Nasal Mucosa, Mesenchymal Stem Cells, Calcium Phosphates;, Cell Differentiation, Tissue Engineering

CLC Number:

R394.2

Shi Wen-tao, Dai Yao, Chen Ping-bo, Bi Shi-qi, Lü De-ming, Lu Hao, Zhang Zhi-jian. Compatibility of nasal mucosa-derived ectomesenchymal stem cells and calcium phosphate cement in vitro[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5350-5355.

Figures/Tables 4

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